海洛因依赖对大鼠胃窦胃泌素、生长抑素阳性细胞的影响

EFFECTS OF HEROIN DEPENDENCE ON Gas,SS POSITIVE CELLS IN GASTRIC ANTRUM OF RATS

目的探讨海洛因依赖对大鼠胃窦胃泌素(Gas)、生长抑素(SS)阳性细胞的影响,以及Gas、SS在海洛因依赖时的可能作用。方法应用免疫组织化学SABC法和图像分析技术。结果1.与正常组和盐水对照组相比,海洛因依赖大鼠胃窦内的Gas阳性细胞数量增加(P<0.05),染色强度随依赖时间逐渐增强,平均灰度值明显低于空白及盐水对照组(P<0.05),且随依赖时间的延长呈下降的趋势,38d时最低。2.海洛因依赖组大鼠胃窦SS阳性细胞染色强度增强,细胞数量显著高于空白和盐水对照组(P<0.05),平均灰度值低于空白及盐水对照组(P<0.05)。结论大鼠胃窦Gas、SS阳性细胞可能通过增加细胞数量和增加Gas、SS的合成来参与大鼠海洛因依赖的调节过程。

Objective To explore the effects of heroin dependence on the gastrin（Gas）, somatostatin（SS） positive cells in gastric antrum of rats and comprehend the possible functions of Gas, SS during heroin dependence. Methods lmmunohistochemical SABC method and imagine analysis technique were employed. Results 1. Compared with the normal control group（NCG） and saline control group（SCG）, the number of Gas positive cells increased （P 〈 0.05）, the intensity of staining stepped up gradually with the passage of time during dependence. The results of imagine analysis showed that the mean grey degree of Gas positive cells had the trend of descent, and it was lower than that in the NCG and SCG with the lowest degree on the 38th day. 2. The intensity of staining and the number of SS positive cells in gastric antrum in heroin dependence group （HDG） increased obviously compared with that in NCG and SCG（ P 〈 0.05） during heroin dependence, the mean grey degree stepped down remarkably than that in NCG and SCG. Conclusion The Gas, SS positive cells in gastric antrum of rats participate in the regulation course during heroin dependence by increasing the cell number and enhancing Gas and SS synthesis.