兴安落叶松胚性愈伤组织诱导影响因子的研究

Factors Affecting Induction of embryogenic callus of Larix gmelinii

以成熟和未成熟合子胚为外植体,研究影响兴安落叶松(Larix gm elinii)胚性愈伤组织诱导的几种主要因子。结果表明兴安落叶松合子胚带胚乳培养有利于胚性愈伤组织的诱导;内蒙沙地种源成熟合子胚的诱导率显著(p<0.05)高于加格达奇山地种源;冷藏处理可以提高成熟合子胚胚性愈伤组织的诱导率;不同发育时期的未成熟合子胚的诱导率存在显著差别(p<0.05),其中以子叶初期合子胚(7月5日)诱导率最高;2,4-D对胚性愈伤组织诱导的影响较大,且与BA、KT存在一定的协同作用;S培养基比DCR和MS培养基更有利于胚性愈伤组织的诱导;培养基中琼脂含量为4 g.L-1时,诱导率较高。

Immature and mature zygotic embryos of Larix gmelinii were as explants to induce embryogenic callus. Several factors influencing the induction were assayed. The induction rate （IR） of embryogenic callus（EC） from zygotic embryo（ZE） explant with its endosperms was higher than that from ZE explant without its endosperms. The IR of EC from mature ZE explant from Inner Mongolia seed source was significantly higher than that which from Daxing＇ an Mountains seed source （p 〈 0.05 ）. The IR of EC from mature ZE explant derived from seeds stored at 4℃ for two months could be improved evidently. The IR of EC from immature ZE explants derived from seeds collected in different developmental stages was significant different, highest IR of EC could be obtained from immature ZE explant collected on July 5. 2,4-D had greater effects on EC induction than BA and KT, and these three hormones had certain co-efficient effects. S medium was more effective than MS and DCR media. Higher IR could be obtained on medium with 4 g·L^-1 agar.