短柄枹种群遗传多样性的ISSR分析

Genetic Diversity of Quercus glandulifera var.brevipetiolata Populations Revealed by ISSR Markers

利用ISSR分子标记方法对分布在浙江省境内的7个短柄枹种群的遗传多样性和遗传分化进行了分析。从100个引物中筛选出12个用于正式扩增的ISSR引物,在7个种群140个个体中共检测到132个位点,其中多态位点118个,多态位点百分率(P)为89.39%,各种群P平均为58.87%。短柄枹总的Shannon信息指数(I)为0.493 3、Nei指数(h)为0.334 7,各种群I平均为0.336 2、h平均为0.229 1。P、I、h均显示云峰种群最高,天台山种群最低。AMOVA分子差异分析表明,67.97%的变异存在于种群内,32.03%的变异存在于种群间,种群间的基因分化系数(GST)为0.315 4。短柄枹种群间的基因流为(Nm)为1.085 3。7个种群的平均遗传距离为0.173 9。利用UPGMA法对7个种群进行聚类,结果显示天台山和雪窦山种群聚成一类,其它5个种群聚成另一类。

Using inter-simp tion of Quercus glandulifera le sequence repeat var. brevipetiolata （ISSR） makers, genetic diversity and genetic differentia- populations, which distribute in Zhejiang Province, were analyzed, A total of 132 bands were amplified from 7 populations with 140 individulas by 12 reliable primers, which was selected from 100 primers. Of them, 1.18 bands were polymorphic and the percentage of polymorphic loci of Quercus glandulifera var. brevipetiolata species was 89. 39%. The percentage of polymorphic loci of Quercus glandulifera var. brevipetiolata populations averaged 58.87%. Shannon information index （I） and Nei＇s gene index （h） at species level were 0. 493 3 and 0. 334 7, respectively, while those at population level averged 0. 336 2 and 0. 229 1, respectively. It suggested that the genetic diversity of YF population was the highest while that of TTS population was the lowest. Analysis of molecular various showed that 67.97% of the total variation was distributed among populations, while only 32.03% was distributed among individuals within populations. The gene differentiation coefficient （GST） among populations is 0. 315 4. The gene flow among populations is 1. 085 3. The average genetic distance of 7 populations was 0. 173 9. Using unweighted pair group method arithmatic average （ UPG- MA）, TTS population and XDS population were clustered into one group and the other five populations were clustered into another group.