转UGPase基因喜树木材化学成分与生长速率研究(英文)

Wood Chemical Compositions and Growth Rate Analysis of UGP Transgenic Camptotheca acuminata

分析了田间栽培条件下2年生转UGPase基因喜树与对照株的木材化学成分与生长速率。结果表明,转UGPase基因喜树综纤维素含量达到78.87%,比对照株相比提高了2.23%;纤维素含量为36.34%,与对照株相比没有明显提高;木质素含量为15.05%,较对照株降低了1.75%;两者的灰分含量均较低且无显著差异;冷、热水抽提物含量为7.62%与10.17%,分别提高了2.04%与2.13%;1%NaOH抽提物含量为27.13%,提高了1.27%。因此,就综纤维素、木质素、灰分含量而言,转UGPase基因喜树为优质纸浆材,水抽提物和1%NaOH抽提物的含量略高,在纸浆生产中需加以重视。本文还对转UGPase基因喜树与对照株的株高、基径、生物量进行了动态监测,结果表明,从5月25日到11月10日的生长季中,其株高平均增加121 cm,对照株平均仅67.8 cm,株高生长速率提高了78.47%;基径平均增加1.792 cm,对照株平均仅0.532 8 cm,提高了236.37%;地上部分生物量的积累与对照相比提高了322.61%,即转入UGPase基因使喜树生长速率显著提高。因此,虽然转UGPase基因喜树的综纤维素和纤维素含量没有明显提高,但其生长速率快,生物量增长显著,间接提高了纤维素与喜树碱的产量。因此,转基因喜树较普通喜树更符合纸浆材速生、纤维素含量高和产量高、木质素含量低的基本要求,可在生产中进一步推广。

Plant UDP-glucose （UDPG） pyrophosphorylase （UGPase） is involved in the production of UDPG, a key metabolite for sucrose and cell wall biosynthesis. It has been shown that UGP can increase the cellulose content of wood. In order to enhance the cellulose content of Camptotheca acuminata Decne, Axgllnum UGP gene had been transferred into C. acuminata. This paper concerned the wood chemical compositions and growth rate of the UGP transgenic C. acuminata, analyzed the data according to the typical broad-leaved pulpwood stander. Result showed that ： the contents of holocellulose, cellulose, and lignin were 78.87%, 36.34% and 15.05% respectively, the transformed plants are more fitful for paper making compared with control. From May to November, we checked the height, base diameter and biomass of UGP transgenic plant and control each month, the result showed that： three parameter of transgenic plants raised 78.00%, 236.37% and 322.61% respectively of control. In short, the growth rate of transgenic plant increased obviously. Above all, C. acuminata was a good quality pulpwood, and the UGP, although not increasing the cellulose properties obviously, improved the growth rate and total biomass significantly, and increased the cellulose yield indirectly.