摘要
本研究采用免疫金银染色和相差显微镜(IGSS/RCM)检测了73份早期钩体病人血涂片。阳性9份,阳性率12.3%。其中发病3日内的钩体病人血标本45份,阳性率17.8%,与血培养阳性率相当,显著高于显微镜凝集试验阳性率6.7%。结果表明,IGSS/RCM是一种较为理想的钩体病早期诊断方法,较血培养和MAT简单、快速、灵敏。如果操作仔细,还可半定量,估计到病人感染钩端螺旋体的强度。
73 blood samples collected from patients were detected by immuno Gold Silver Staining and Reflection Contrast Microscopy (IGSS/RCM).9 of these samples were positive by IGSS/RCM.Positive rate was 12.3%.Of 45 amples from those patients ,their onset dates were within 3 days,8 samples were positive and positive rate was 17.8% by IGSS/RCM.There was no difference between the rates by using this mothod and blood culture method,but was higher than Microscopy Agglutination Test(MAT).The authors suggested that IGSS/RCM was an ideal method for early diagnosis of Leptospirosis.In contrast with culture and MAT,it was simple,rapid and sensitive.
出处
《疾病监测》
CAS
1997年第2期63-65,共3页
Disease Surveillance
基金
欧共体资助
关键词
钩端螺旋体病
免疫金银染色
相差显微镜
诊断
Immuno Gold Silver Staining Reflection Contrast Microscopy, Leptospirosis