应用RAMP标记分析水貂与彩貂种属间的遗传多样性

Genetic Diversity among Mink Based on the RAMP Markers

对标准黑褐色水貂、蓝宝石貂、咖啡色貂3个品种的89个个体进行了RAMP分析。结果表明,品种间遗传差异明显,多态性高。14个引物组合扩增出的81条DNA片段中,75条具有多态性,多态比例为92.59%,每个引物组合可扩增4-8条多态性条带,平均5.78条。多态条带比率、Shannon多样性指数以及Nei氏基因多样度（h）均显示水貂与彩貂品种较为丰富的遗传多样性。Nei遗传距离、Gst基因分化系数显示水貂与彩貂品种间遗传分化程度不高,遗传变异主要来自种群内。

Relationships among three mustela vison species which were standard-pitchy mink, sapphire mink,coffee mink were analyzed by RAMP Markers. The results showed distinct genetic differences were present among the species, and polymorphic percentage was very high. Fourteen primer combinations produced 81 bands, 75 bands were polymorphic. The polymorphic percentage was 92.59%, 4-8 bands were amplified by each primer combinations, with an average of 5. 78 bands. Polymorphic bands percent .Shannon＇s Information index,Nei＇s （1973） gene diversity showed high genetic diversity. In population genetic differentiation analysis, Nei＇s genetic distance and the coefficient of gene differentiation were used and the results showed that the genetic differentiation degree between populations was lower and the hereditary variation primarily came from within population.