国标法对乳碱性磷酸酶的检测及其在巴氏消毒上的研究

The Study on the Determination of Alkaline Phoaphate by the National Standard in Milk and the Application in the Pasteurization

采用国标法对乳中碱性磷酸酶(ALP)检测,结果显示:全乳和脱脂乳的酶活力分别为403.345、148.8147μg/ml,变异系数分别为1.30%、2.99%。与国标法的不同之处:乳样最佳稀释比为1∶25,苯酚的最佳浓度为50μg/ml;然后对其模拟巴氏消毒得出:63℃30 min基本使ALP灭活。

The result of using the national standard to measure the alkaline phosphate （ALP）in milk is as follows： the activity of enzyme in the whole milk and skimmed milk is 403. 345μg/ml, 148. 8147 μg/ml respectively; the coefficient of variation is 1.30 %, 2.99 %, respectively. All these point this method is good and stable. The difference compounded to the national standard： the best dillution ratio of milk samples is 1 ： 25, the right consistency of phenol is 50 μg/ml. The conclusion by simulating pasteurization： ALP is inactived at 63℃for 30 min.