硫化氢改良的St.ThomasⅡ液保存离体大鼠心脏不同保存时限超微结构的变化

Ultrastructural change of isolated rat hearts after hypothermic preservation by St.Thomas Ⅱ solutions containing H_2S

目的探讨含硫化氢(H2S)的St.ThomasⅡ液,对离体大鼠心脏低温下不同保存时间再灌注30 min后,心肌超微结构的变化。方法SD大鼠32只随机分成4组,每组8只,分别为:低温保存4 h组,低温保存6 h组,低温保存8 h组,低温保存10 h组。停搏与保存均用含硫化氢(H2S)的St.ThomasⅡ液进行低温保存。采用Langendorff心脏灌注和工作模型装置,进行心脏保存前及保存后再灌注30 min心功能测定,比较心功能恢复率、心肌酶(CK,LDH)漏出量,以及超微结构心肌组织变化。结果随着保存时间的延长心功能恢复率逐渐降低,心肌酶漏出量逐渐增加。在保存4 h和6 h后心肌细胞出现轻度损伤,在保存8 h和10 h后,心肌细胞呈现中度损伤,但保存10 h后的离体心脏再灌注后心功能的恢复率与保存8 h后相比较存在显著的差异。结论用含H2S的St.ThomasⅡ液低温保存大鼠离体心脏,8 h和10 h后心肌组织均出现中度损伤,保存10 h再灌注后出现严重的心功能障碍,而保存8 h以内的离体心脏的保存效果良好。

AIM To investigate the uhrastructural changes of isolated rat hearts after hypothermic preservation by St. Thomas Ⅱ solutions containing H2S for different periods of time and reperfusion for 30 minutes. METHODS Thirty-two isolated rat hearts were randomly divided into four groups （ n = 8 each） and the hearts were arrested with St. Thomas Ⅱ plus hydrogen sulfide solution and preserved at 4℃ hypothermically in the same solution for 4 h, 6 h, 8 h and 10 h. The isolated hearts were perfused on the Langendorff perfusion apparatus for functional evaluation before and after preservation. Myocardial enzyme leakage and cardiac uhrastructure were measured after reperfusion. RESULTS The recovery of myocardial function decreased with the extension of preservation time and myocardial enzyme leakage increased gradually. Cardiac uhrastructure presented a minor injury after the hearts were preserved for 4 h and 6h and presented secondary injury after 8 h and 10 h. After reperfusion, the difference of the recovery of myocardial function was significant between 8 h group and 10h group. CONCLUSION When isolated hearts are preserved in St. Thomas Ⅱ solutions containing H2S, the hearts could be well preserved within 8 h preservation. Secondary injury of myocardium tissue would appear after 8 h and 10h preservation and the function of the heart would be seriously impaired after 10h preservation.