锌和某中草药制剂拮抗铅毒作用的实验研究

Experimental Study on Antagonization of Lead Toxicity by Zinc and Chinese Herbal Preparation

目的研究锌和某中草药制剂对铅染毒小鼠某些生化指标的影响。方法选取60只健康雄性昆明种小鼠随机分成5组:治疗1组、治疗2组、治疗3组、阳性对照组、阴性对照组,每组12只。前4组用2 g/L醋酸铅水溶液腹腔注射染毒,1次/d,连续染毒35 d。阴性对照组腹腔注射等容积的生理盐水。染毒14 d后,开始灌胃给药治疗。治疗1组单独给予锌、治疗2组给予锌加中草药制剂、治疗3组单独给予中草药制剂,阳性对照组和阴性对照组灌胃给予等量的蒸馏水,1次/d,直至实验结束。实验开始和实验结束均称量各实验组小鼠体重并测定血红蛋白(Hb)。实验结束后,测定各实验组小鼠血清铅、锌、钙、尿素和丙氨酸氨基转移酶(ALT),测定脑钙、NO含量、乙酰胆碱酯酶(TchE)活力、谷胱甘肽过氧化物酶(GSH-Px)活力和脑、肝匀浆对超氧阴离子(O2.)清除率。结果治疗1组、治疗2组、治疗3组、阳性对照组和阴性对照组血铅分别为(0.070 9±0.012 9),(0.052 2±0.051 8),(0.054 7±0.017 5),(0.119 0±0.027 2),(0.010 3±0.002 6)mol/L,阳性对照组明显高于其他各组;血清锌分别为(0.096 8±0.038 5),(0.084 9±0.010 3),(0.091 1±0.031 8),(0.023 5±0.037 2),(0.061 8±0.003 1)mol/L,阳性对照组明显低于治疗2组和治疗3组;脑NO分别为(13.66±3.43),(12.85±4.70),(15.80±4.40),(23.03±7.80),(12.59±3.90)μmol/L,阳性对照组明显高于其他各组;脑钙分别为(1.9±0.1),(1.0±0.2),(1.1±0.2),(0.6±0.2),(2.2±0.3)mol/L,阳性对照组明显低于其他各组;脑TchE分别为(2.67±0.69),(5.95±1.59),(6.08±1.65),(6.21±0.28),(2.86±0.99)U/mg蛋白,阴性对照组和治疗1组明显低于其他各组;其余指标,血清ALT、尿素、钙含量、Hb、O2.清除率,阳性对照组与阴性对照组、治疗组之间均有明显差异。结论锌和某中草药制剂对铅染毒小鼠部分生化指标有明显的促进恢复作用。

Objective To study the effect of zinc and Chinese herbal preparation on biochemical indices in lead exposed mice. Methods 60 male mice were divided randomly and equally into five groups, namely treatment group 1, treatment group 2, treatment group 3, the positive control group and the negative control group. Mice in former 4 groups were exposed to lead by peritoneally injecting 2 g/L lead acetate water solution once a day successively for 35 days. Mice in the negative control group were peritoneally injected with normal saline at the same volume. After being exposed for 14 days, the treatment began. Mice in the treatment group 1 were given zinc only. Mice in the treatment group 2 were given zinc and Chinese herbal preparation. Mice in the treatment group 3 were given Chinese herbal preparation only. Mice in the positive control group and negative group were given equal amount of distilled water. All these were done once a day till to the end of experiment. Body weight and Hb of each mouse were measured before and after the experiment. After the experiment was ended, levels of lead, zinc, calcium, urea and ALT in serum, levels of calcium, NO, activity of TchE and activity of GSH- Px in brain tissues, clearance of brain homogenate and liver homogenate to O2- were determined. Results Serum lead Levels in treatment groups 1, 2 and 3, and the positive and negative control groups were（0.070 9±0.012 9）, （0.052 2±0.051 8）, （0.054 7± 0.0175）, （0.1190±0.0272）, （0.0103±0.0026） mol/L, in which the content in the positive control group was obviously higher than those of other groups. Serum zinc levels were （0. 096 8 ± 0. 038 5）, （0. 084 9 ± 0.010 3）, （0.091 1±0.031 8）, （0.023 5±0.037 2）, （0.061 8±0.003 1）mol/L, in which the content in the positive control group was obviously lower than those in treatment groups 2 and 3. Brain NO were（13.66 ±3.43）, （12.85±4.70）, （15.80±4.40）, （23.03±7.80）, （12. 59±3. 90）μmol/L, in which the content in the positive control group was obviously higher than those of other groups. Brain calcium levels were（1.9 ± 0.1）, （1.0 ±0.2）, （1.1±0.2）, （0.6±0.2）, （2.2±0.3）mol/L, in which the content of the positive control group was obviously lower than those of other groups. Brain TchE levels were（2. 67±0.69）, （5. 95± 1.59）, （6.08±1.65）, （6.21±0.28）, （2.86±0.99）U/mg protein, the contents in the negative control group and treatment group 1 were obviously lower than those of other groups. As to other indexes such as serum ALT, urea, calcium content, Hb, and clearance of O2, significant differences existed among the positive control group and the negative control group, and all treatment groups. Conclusion There is a significant promotion effect to recover some biochemical indices of lead exposed mice by zinc and Chinese herbal preparation.