转化生长因子-β基因多态性与尘肺相关性研究

Study on Association of Gene Polymorphism of Transforming Growth Factor-β with Pneumoconiosis

目的探讨转化生长因子-β(TGF-β)基因多态性与尘肺的关系。方法采用1∶1配对的病例对照研究方法,尘肺组为确诊的117例Ⅰ期尘肺病人,对照组为未患尘肺的接尘工人。应用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)技术对TGF-β启动区的-509C/T位点和第一外显子的+869T/C、+915G/C位点的基因多态性进行检测。结果TGF-β启动区的-509C/T位点在尘肺组中的TT基因型频率高于对照组,差异有统计学意义(P<0.05),-509*T等位基因频率也高于对照组,差异有统计学意义(P<0.05);+869T/C位点等位基因和基因型频率与对照组比较,差异均无统计学意义(P>0.05);在尘肺组中+915G/C位点的GC基因型频率高于对照组,差异有统计学意义(P<0.05),+915*C等位基因频率高于其对照组,差异有统计学意义(P<0.05)。结论TGF-β-509TT和+915GC基因型是尘肺的易感基因型;同时携带TGF-β-509*T和TGF-β+915*C等位基因的接尘人群更易感尘肺。未发现TGF-β+869位点基因多态性与尘肺易感性有关联。

Objective To explore the association of genetic polymorphisms of TGF-β with pneumoconiosis. Methods One to one pair matching case-control study was carried out. 117 pneumoconiosis patients diagnosed as stage Ⅰ were selected as case subjects, and dust exposed healthy workers as the control group. Using polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP）, gene polymorphisms of TGF-β promoter-509C/T, and ＋869T/C and＋915G/C in exon 1 were measured. Results The frequencies of this TGF-β-509C/T TT genotypes for pneumoconiosis was significantly higher than the control group. The differences had statistical significance （ P〈0. 05 ）. -509 ＊ T alleles for pneumoconiosis were also significantly higher than the control group. The differences had statistical significance（P（0.05）. There was no significant difference in the frequency of TGF-β ＋ 869 genotypes and allelic between pneumoconiosis and control（P〉0. 05）. The frequencies of this TGF-β＋ 915 GC genotypes for pneumoconiosis was significantly higher than the control group. The differences had statistical significance（P〈0. 05）. ＋ 915 C alleles for pneumoconiosis ware significantly higher than the control group. The differences had statistical significance（P〈0.05）. Conclusions TGF-β -509TT genotype and ＋915GC genotype may be a susceptible factor to pneumoconiosis. The dust exposed worker carrying TGF-β-509 ＊ T and TGF-β＋915＊C alleles are more susceptible to pneumoconiosis. The findings suggest that the ＋869C polymorphisms of the TGF-β gene are not associated with the pneumoconiosis.