原发性高血压患者血浆脂肪酸的研究

Plasma fatty acid analyses of essential hypertension

目的:探讨原发性高血压患者血浆脂肪酸组成及含量情况。方法:(1)试验分2组,正常对照组选取正常健康者60例。高血压组选取单纯原发性高血压患者56例,采用Morrison法提取血浆脂肪酸,以气相色谱法检测三氟化硼甲脂化的脂肪酸,计算出各组分所占重量百分比。结果:血浆脂肪酸测定结果高血压组与正常对照组比较差异有统计学意义(P<0.05)(1):高血压组血浆总饱和脂肪酸(SFA)增高,其中增高明显的SFA为豆蔻酸、棕榈酸和硬脂酸。(2)单不饱和脂肪酸(MUFA):高血压组的反式油酸明显增高,油酸则较正常对照组明显减少。(3)多不饱和脂肪酸(PUFA):①总PUFA在高血压组明显减低;②n-6PUFA中:高血压组的亚油酸,Υ-亚麻酸,花生四烯酸,二十二碳四烯酸和二十碳三烯酸均较正常对照组低,尤以二十二碳五烯酸降低更明显;③n-3PUFA中:高血压组的α-亚麻酸、二十碳五烯酸及二十二碳六烯酸均明显减少(均P<0.05)。结论:原发性高血压患者血浆中不但豆蔻酸、棕榈酸和硬脂酸及反式油酸较正常人明显增高,而且油酸、亚油酸,α-亚麻酸及二十碳五烯酸及二十二碳六烯酸均较正常人明显降低,Υ-亚麻酸、花生四烯酸、二十碳三烯酸、二十二碳四烯酸和二十二碳五烯酸也较正常人明显缺乏。

Objective：To explore the plasma fatty acid concentration in cases of essential hypertention（EH）. Method： Plasma samples were collected from 56 patients with EH, 60 normal people as controls. Plasma total fatty acid concentration was analyzed with Morrison method and ran in 25-meter capillary gas-liquid chromatography. Result： Of the major fatty acid classes, （1） the percentage of C14：0, C16：0 ,C18：0 and total saturated were significanly higher in E H group than in control group, （2）The percentage of C18：1tr （trans oleic acid） was significantly higher in EH group than in control group. But the percentage of C18：1（oleic acid ）was significantly lower in EH group than in control group. （3） Total polyunsaturated fatty acid,：① total n-3 and total n-6 polyunsaturated were lower in EH group than in control group . ②the percentage of C18： 2 n-6 （Linoleic acid）, C18：3 n-6 （T-linic acid）, C20：4 n-6（Arachidonic acid-AA）,C22：4 n-6,C20：3 n-6;C20：5 n-6 in EH group were significantly lower than in control group;. Qthe percentage of C20：5 n-3（EPA） and C22：6 n-3（DHA） and C18：3 n-3 （a-linic acid）, in EH group were significantly lower than in control group. Conclusion： Our datum demonstrated that the plasma fatty acid of EH was abnormal not only the percentage of C14：0, C16：0,C18：0, total saturated fatty acid and C18：1tr （trans oleic acid） were significantly higher than in control group, but also the percentage of C18：2 n-6 （Linoleic acid） , C18：3 n-3（a-linic acid） ,C20：5 n-3（EPA） and C22：6 n-3（DHA） were significantly lower than in control, it is more important that we found the percentage of C18：3 n-6（T-linic acid ）, C20：4 n-6（Arachidonic acid-AA） group,C20：3 n-6, C22：4 n-6 also significantly lower than that of control group.