14例乙肝患者肝组织HBV抗原表达与肝脏病变和血清HBVDNA的关系

RELATIONSHIP BETWEEN HBV ANTIGEN EXPRESSION IN HEPATIC TISSUE AND HEPATIC LESIONS OR SERUM HBVDNA IN 14 CASES OF HEPATITIS B

刮用敏感的多聚酶链反应(PCR)技术及免疫组化方法,对14例乙肝患者的血清HBVDNA、肝组织 HBsAg 和 HBcAg 分别进行了检测。结果显示:HBeAg 阳性不能完全反应HBVDNA 复制的存在。慢迁肝较怎性肝炎和慢活肝的肝组织内 HBsAg 和 HBcAg 检出率高。肝组织内 HBV 抗原的检测可做为乙肝病原诊断的依据。

Using the sensitive PCR techniques and immunohistochemical method, we detected serum HBVDNA and hepatic tissue HBsAg and HBcAg in 14 patients with hepatitis B.The results show that HBeAg positivity can not completely reflect the presence of HBVDNA replication.Compared with acute hepatitis and chronic active hepatitis,the positivity rate of HBsAg and HBcAg in hepatic tissue from chronic persisting hepatitis was high.The detection of HBV antigens in hepatic tissue may provide an important basis for etiological diagnosis of hepatiris B.