摘要
目的:用HPLC法对龙血竭指纹图谱进行研究。方法:采用色谱条件Eclipse XDB-ODS柱(4.6 mm×150 mm,5μm);流动相乙腈-水,梯度洗脱进行色谱分离;检测波长为275 nm;流速为1.0 mL.min-1;柱温为40℃,以龙血素B作为参照物。结果:初步建立了龙血竭的HPLC指纹图谱,并进行相似度评价。结论:方法简单可行,能够有效地控制龙血竭的内在质量。
Objective： To study the fingerprint of dragon＇ s blood resina draconis by high performance liquid chromatography. Method： The samples are extracted with methanol and separated on a Eclipse XDB-C18 column （4. 6 mm× 150 mm, 5 μm） with the mobile phase of acetonitrile-H20 in gradient mode, and the flow rate was 1.0 mL·min^-1, the detection wavelength was 275 nm and the temperature of column was 40 ℃. Loureirin B was used as the reference compound. Result： HPLC fingerprint of dragon＇ s blood was established and the similarity of the fingerprint was compared. Conclusion： The method is simple, accurate, and can be used to control the quality of dragon＇ s blood.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第19期2025-2027,共3页
China Journal of Chinese Materia Medica
