酶联免疫吸附法检测病毒性肝炎患者血清白细胞介素12和肿瘤坏死因子的水平

Determination the serum interleukin-12 and tumor necrosis factor in patients with viral hepatitis by enzyme linked immunosorbent assay

目的测定白细胞介素12(IL-12)与肿瘤坏死因子(TNF)在病毒性肝炎患者血清中浓度。方法用双抗体夹心酶联免疫吸附法检测64例病毒性肝炎患者及20例正常对照者的血清IL-12与TNF水平。结果病毒性肝炎患者血清IL-12与TNF水平明显高于正常对照组(P<0.01);血清IL-12与TNF水平与血清总胆红素及谷丙转氨酶水平呈正相关。结论IL-12及TNF参与了病毒性肝炎的免疫病理过程,早期检测血清IL-12与TNF水平,可判断病情的严重程度及预后。

Objective To determination the serum interleukin- 12 （IL -12） and tumor necrosis factor （TNF） in patients with viral hepatitis. Methods The levels of IL - 12 and TNF were measured by enzyme linked immunosorbent assay in 64 patients with viral hepatitis and 20 healthy adults subject as control. Results The levels of IL - 12 and TNF were significantly higher in patients with viral hepatitis than those in healthy control（P 〈0.01 ）. The serum IL - 12 and TNF levels were positively correlated with serum total bilirubin and alanine aminotransferase levels. Conclusion IL - 12 and TNF participates in the activity process of viral hepatitis. Levels of serum IL - 12 and TNF in early stage may reflect the severity of viral hepatitis and be helpful to prognosticating the disease.