光动力学疗法对兔耳增生性瘢痕作用的初步研究

Effect of HMME-PDT on hyperplastic scar in rabbit ear model

目的 探讨光动力学疗法对兔耳增生性瘢痕的作用。方法 建立兔耳增生性瘢痕模型后，将增生性瘢痕块随机分为血卟啉单甲醚（HMME）一光动力学疗法（PDT）治疗组和对照组（n=12）。观察瘢痕生长情况，建模术后28d取材行常规HE染色和胶原纤维VG染色，计算微血管密度，观察PDT对瘢痕的影响。结果 PDT治疗后瘢痕厚度显著降低，真皮层变薄；微血管数量及成纤维细胞数量减少，且主要位于真皮深层；胶原含量下降，胶原纤维排列较规则有序。结论 在瘢痕形成早期，HMME—PDT能够有效抑制兔耳瘢痕的增生，该方法有可能成为瘢痕预防与治疗的有效方法。

Objective To investigate the effect of HMME-PDT（ Hematoporphyrin Monomethyl Ether- Photodynamic therapy ） on Hyperplastic scar in the rabbit ear. Methods The acute model of dermal Hyperplastic scar in the rabbit ear was established. 24 scars were randomly divided into 2 groups： the experimental group （n = 12） received HMME-PDT treatment, and the controlled group （ n = 12） received no special treatment. Specimens were harvested from scars on postoperative 28 day. Scar hyperplasty and collagen fibers were observed by haematoxylin-eosin staining and Van-Gieson staining respectively. The microvessel density was calculated under microscope. Results Compared with the controlled group, HMME-PDT treatment in the experimental group reduced scar formation, decreased the microvessel density and prevented excess collagen deposition at the wound site. Conclusions HMME-PDT may play a role in inhibiting hyperplastic scar in rabbit ear.