摘要
目的 探讨光动力学疗法对兔耳增生性瘢痕的作用。方法 建立兔耳增生性瘢痕模型后,将增生性瘢痕块随机分为血卟啉单甲醚(HMME)一光动力学疗法(PDT)治疗组和对照组(n=12)。观察瘢痕生长情况,建模术后28d取材行常规HE染色和胶原纤维VG染色,计算微血管密度,观察PDT对瘢痕的影响。结果 PDT治疗后瘢痕厚度显著降低,真皮层变薄;微血管数量及成纤维细胞数量减少,且主要位于真皮深层;胶原含量下降,胶原纤维排列较规则有序。结论 在瘢痕形成早期,HMME—PDT能够有效抑制兔耳瘢痕的增生,该方法有可能成为瘢痕预防与治疗的有效方法。
Objective To investigate the effect of HMME-PDT( Hematoporphyrin Monomethyl Ether- Photodynamic therapy ) on Hyperplastic scar in the rabbit ear. Methods The acute model of dermal Hyperplastic scar in the rabbit ear was established. 24 scars were randomly divided into 2 groups: the experimental group (n = 12) received HMME-PDT treatment, and the controlled group ( n = 12) received no special treatment. Specimens were harvested from scars on postoperative 28 day. Scar hyperplasty and collagen fibers were observed by haematoxylin-eosin staining and Van-Gieson staining respectively. The microvessel density was calculated under microscope. Results Compared with the controlled group, HMME-PDT treatment in the experimental group reduced scar formation, decreased the microvessel density and prevented excess collagen deposition at the wound site. Conclusions HMME-PDT may play a role in inhibiting hyperplastic scar in rabbit ear.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2007年第5期425-427,共3页
Chinese Journal of Plastic Surgery
关键词
光动力学疗法
增生性瘢痕
微血管
胶原
Photodynamic therapy
Hyperplastic scar
Microvessel
Collagen