热休克蛋白HSP70-2＋1267（A／G）位点基因多态性与强直性脊柱炎相关性研究

The heat shock protein 70-2 gene＋ 1267（A/G） polymorphism and susceptibility to ankylosing spondylitis in Chinese Han population

目的探讨热休克蛋白HSP70—21267（A／G）位点基因多态性与强直性脊柱炎发生的关系。方法采用聚合酶链式反应-限制性片段长度多态性方法检测了176例强直性脊柱炎（ankylosing spondylitis．As）患者和127名正常人的HSP70-21267（A／G）位点基因多态性。结果AS患者HSP70-2各基因型（AA，AG，GG）频率分别为46．6％、46．0％和7．4％，在对照者中分别为44．1％，48．8％和6．9％两组之间的基因型频率分布的比较，差异没有统计学意义（X^2=0．232，P〉0．05）；A和G的等位基因频率在AS中分别69．6％（A）和30．4％（G），在对照者中分别为68．5％和31．5％，两者之间比较，差异没有统计学意义（X^2=0．083，P〉0．05）。结论HSP70-21267（A／G）基因多态性位点可能不是中国人患AS的主要易感位点基因。

Objective To investigate the association between the heat shock protein 70-2 gene polymorphism and ankylosing spondylitis （AS）. Methods The polymorphisms of HSP70-2 gene Pst I 1267 site were analysed in 176 Chinese Han AS patients and 127 healthy controls by PCR and restriction fragment length polymorphisms（RFLP） meth- ods. Results In AS patients HSP70-2 genotypes AA, AG and GG were 46.6%, 46.0% and 7.4% respectively, frequencies of A and G were 69.6%（A） and 30.4% （G）. In healthy controls HSP70-2 genotypes AA, AG and GG were 44.1%, 48.8 % and 6.9% respectively, frequencies of A and G were 68.5 % （A） and 31.5 % （G）. No significant differences were found in the distribution of HSP70-2 genotypes and allele frequencies between AS patients and controls. Conclusion Our results indicate that the there may be no association of the HSP70-2 gene polymorphism with AS in Chinese Han population.