中国苏州地区汉族人群MCP-1基因多态性与急性胰腺炎相关性研究

Relationship between genetic polymorphism of MCP-1and acute pancreatitis in Han population of Suzhou in China

目的探讨中国苏州地区汉族人群单核细胞趋化因子蛋白．1（monocyte chemoattractant protein-1，MCP-1）基因-2518A／G多态性与急性胰腺炎的相关性。方法采用聚合酶链反应．限制性片段长度多态性方法检测中国苏州汉族人群急性胰腺炎（acute pancreatitis，AP）患者101例[其中包括轻症急性胰腺炎（mildAP，MAP）78例，重症急性胰腺炎（servere AP，sAJP）23例]和120名健康对照的MCP-1基因-2518位点基因型分布及基因频率，并评价基因多态性与AP易感性之间的相关性。结果对照组MCP-1—2518A／G位点的从基因型频率较SAP组和MAP组高，差异有统计学意义（P〈0．01），携AG及GG基因型者患MAP的风险度约是从基因型的5．896倍（P〈0．01，OR=5．896），患SAP的风险度约为7．011倍（P〈0．05，OR=7．011）。而在MAP与SAP组间从基因型频率差异无统计学意义（P=0．997）。对照组G等位基因频率明显低于MAP（P〈0．01，OR=0．318）和SAP组（P〈0．01，OR=0．309）。但G等位基因频率在MAP与SAP组间基因型分布差异无统计学意义（P=0．623，OR=1．211）。结论中国苏州地区汉族人群MCP-1—2518位点从基因型可能有助于保护机体避免发生AP，G等位基因频率较高的人可能易患AP。但从基因型和G等位基因频率不能预测SAP的发生。

Objective To study the relationship between monocyte chemoattractant protein-1 gene （MCP-1） - 2518A/G polymorphism and acute pancreatitis （AP） in the Han population of Suzhou, China. Methods The polymorphisms were detected with polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. The genotypes and allele frequencies of MCP-1 - 2518A/G were calculated and analyzed in 101 AP patients including 78 mild AP （MAP） patients and 23 severe AP （SAP） patients, and 120 healthy individuals as control group. Results The frequency ofMCP-1 - 2518 AA genotype in control group was significantly higher than that in SAP and MAP groups （ P 〈 0.01 ）. People with AG and GG genotypes had 5.896 times risk of developing MAP （ P 〈 0.01, OR = 5.896） compared with people with AA genotype. Subjects carrying G allele were at a 7-fold elevated risk for SAP （ P 〈 0.05, OR = 7.011） contrasted with subjects carrying AA genotype. However, no difference in AA genotypic distribution was noted between MAP and SAP groups （X^2 = 0.006, P = 0. 997）. The frequency of G allele in healthy controls was obviously lower than that in MAP （ P 〈 0.01,OR= 0.318） and SAP groups （ P 〈 0.01, OR= 0.309）. No difference of G allele frequency was found between SAP group and MAP group （ P = 0.623, OR = 1.211 ）. Conduslon The MCP-1 - 25 18 AA genotype of the population in Suzhou may be a protective genotype of AP. People with higher frequency of G allele is more likely to suffer from AP. Nonetheless, the genotype of AA and the frequency of G allele couldn＇ t predict the risk of SAP.