联合免疫抑制下肝癌复发大鼠模型的建立

Establishment of hepatocellular carcinoma recurrence model in rats under combined immunosuppression regimen

目的建立能反映肝癌肝移植特点的肝癌复发动物模型。方法选用Wistar大鼠，实验组大鼠给予联合免疫抑制方案（MP＋CSA＋MMF），对照组仅同法使用生理盐水，两组均接受经门静脉系统接种Walker-256肿瘤细胞株手术，术后10d处死、剖腹探查。结果实验组手术时间为（26．65±0．42）min，对照组为（26．20±0．30）min，两组间无显著性差异（P〉0．05）；两组大鼠在试验期间均存活；实验组大鼠体重减轻量（56．70±2．74）g较对照组体重减轻量（45．30±2．44）g有显著性差异（P〈0．05）；实验组大鼠肿瘤复发率为95％（19／20），对照组为400（8／20），实验组大鼠肿瘤的复发率明显高于对照组大鼠（P〈0．05）。结论该模型能够有效模拟肝癌肝移植术后肿瘤复发的主要特征，可以作为研究肝癌肝移植术后肝癌复发的大鼠模型。

Objective To establish an animal model of hepatocellular carcinoma recurrence to investigate the recrudescent mechanism after liver transplantation. Methods Thirty Wistar rats were randomized into the immunosuppression group （group A） and control group （group B）. The cell line of walker-256 was injected via portal venous system in both groups. The rats in group A received the combined immunosuppression regimen （MP＋CSA＋MMF）. Normal saline was used in group B. Results There was no significant difference in operative duration between group A and group B （26.65 ±0.42 min/26.20±0.30 min, P〉0.05）. The body weight loss in group A was markedly higher than in group B （56.70±2.74 g/45.30±2.44 g, P〈0. 05）. The survival rate was 100% in both groups. However, the recurring rate was remarkably higher in group A than in group B （95%/40%, P〈0. 05）. Conclusion This model is an optimal clinical model to observe recrudescence that mimics the natural situation of hepatocellular carcinoma in human body after liver transplantation quite well.