子痫前期患者与正常妊娠孕妇羊水差异蛋白质谱分析

Analysis of differential protein mass spectrogram in the amniotic fluid of pre-eclampsia and normal pregnancy

目的 探讨并初步筛选子痫前期患者的特异性羊水标志物。方法 利用CM10蛋白芯片和表面增强激光解析离子化飞行时间质谱（SELDI TOF MS）技术，检测8例重度子瘌前期患者（研究组）和8例晚孕期正常孕妇（对照组）羊水中差异蛋白质的相对含量。并在8例中孕期（孕20±周）正常孕妇（中孕组）羊水中寻找有无这些差异蛋白的存在及其含量变化情况。结果 （1）研究组与对照组羊水蛋白质在相对分子质量／电荷比值（质／荷比）2000-50000范围内，有4种不同质荷比蛋白质的相对含量有明显差异。（2）研究组质荷比为4653的蛋白质相对含量[（1．52±0．71）％]明显低于对照组[（3．16±1．64）％]（P〈0．05），而质荷比为3841、8520、8579的蛋白质相对含量分别为（7．02±4．37）％、（3．74±1．96）%、（5．09±1．93）％，均高于对照组[（2．58±1．09）％、（1．83±0．67）％、（3．06±1．57）％，P均〈0．05]。（3）中孕组羊水中可以找到这四种差异蛋白质。结论 子痫前期患者与正常孕妇羊水蛋白质质谱存在明显差异。有4种不同质荷比相对应的蛋白质可能是子痫前期患者羊水特异性的生物标志物。

Objective To investigate the specific biomarker in amniotic fluid of pre-eclampsia. Methods The relative amount of differential proteins were detected in amniotic fluid of 8 cases of severe pre-eclampsia （study group） and 8 cases of normal pregnancy in late trimester （control group） by CM10 proteinchip array and surface enhanced laser desorption/ionization time of flight mass spectroscopy（SELDI TOF MS） to see whether there were differential proteins and to study the variances of contents in the amniotic fluid of 8 cases of normal pregnancy in mid trimester （mid pregnancy group）. Results （1）Significant differences were found of relative amount of 4 kinds of proteins of different mass/zharge ratio within 2000-50 000 between the study and control group. （2） The relative amount [（1.52±0.71） %] of the protein with mass/zharge ratio of 4653 in the study group was much lower, than that of control[（3. 16±1.64）%] （P〈0.05）. While the relative amount[ （7. 02±4. 37）%, （3.74±1.96）% ,（5.09±1.93）% ]of the proteins which had a mass/zharge ratio of 3841,8520,8579 were higher than those of control[ （2.58±1.09）%,（1.83±0. 67）%,（3.06±1.57）%]（P〈0.05）. （3） Four differential kinds of proteins were found in the mid pregnancy group. Conclusions There are significant difference in the protein mass spectrogram in the amniotic fluid between pre-eclampsia and normal pregnancy. Four different kinds of proteins with different mass/zharge ratio may be the specific biomarkers in the amniotic fluid of pre-eclampsia.