银杏叶提取物及制剂萜类内酯薄层色谱荧光扫描定量测定研究

Study on Assay of Terpenes in the Leaves of Ginkgo biloba Extract and Its Preparations by insitu Fluorometric TLC

建立了采用优化条件展开的薄层色谱进行热化学衍生荧光，原位薄层定量，同板同时测定银杏内酯Ａ，Ｂ，Ｃ及白果内酯，最低检出限分别为０．１２～０．３５μｇ。灵敏度分别为０．２５～０．５μｇ，较ＨＰＬＣ示差折光检测器提高约１０倍，分析时效比提高６倍以上，精密度同板ＲＳＤ２％～３％，异板ＲＳＤ２．３％～３．７％，回收率９５．３％～９８．８％。

A new TLC method for the assay of terpene lactones in Ginkgo biloba extract and its preparations has been established by means of optimized development condition and post chromatographic thermal fluorescence derivatization. Satisfactory results can be obtained through polynomial regression calibration. The data obtained by this method have been proved ten times higher in sensitivity than those obtained by HPLC refracto detector.