生物强化MBR系统处理溴氨酸启动期特性研究

Research on characteristics of bioaugmented MBR treating bromamine acid during startup phase

采用鞘氨醇单胞菌强化膜生物反应器(MBR),对模拟溴氨酸废水进行处理.考查了生物强化MBR启动期溴氨酸的降解、污泥特性及细菌生理状态的变化,并用DNA指纹技术——核糖体基因间区序列分析(RISA)揭示MBR启动期污泥的菌群变化.结果显示,采用生物强化可以使MBR迅速启动并稳定运行.在启动期,污泥浓度下降,沉降性和絮凝性变好;脱氢酶活性下降并稳定在一个较低的水平上;胞外聚合物中蛋白和多糖含量略有上升;启动期末期膜出水溴氨酸脱色率可达90%以上,COD去除率可达60%以上.RISA指纹分析表明污泥系统多样性减少,形成了降解溴氨酸的功能群落.

Sphingomonas xenophaga QYY was used to enhance the ability of membrane bioreactor （MBR） treating simulated bromamine acid wastewater. The degradability and characteristics of activated sludge and the physiological state of microbial community were studied. A DNA fingerprint technique--ribosomal intergenic sequence analysis （RISA） was applied to investigate the changes of microbial community during the startup phase. The results show that bioaugmentation can accelerate the startup of the MBR and relatively maintain stable operation. During the startup phase, the concentration of activated sludge decreased, the settle and flocculation ability was becoming better, the TTC-dehydrogenase activity declined and was kept at a low level, and the concentration of protein and carbohydrate in the extracellular polymeric substances （EPS） was increased. At the end of the startup phase, the decolorization rate of bromamine acid was over 90%, and the removal of COD was over 50%. RISA fingerprint shows that the genetic diversity of the activated sludge communities is decreased. The functional community with the ability of degrading bromamine acid is developed.