热CO_2气腹对结肠癌细胞的增殖抑制作用及其机制

Anti-proliferative effect of hyperthermic CO_2 pneumoperitoneum on colonic cancer cells and its mechanism of action

目的:探讨热CO2气腹对结肠癌细胞的增殖抑制作用及作用机制,明确其用于结直肠癌腹膜转移治疗的可行性。方法:建立热CO2气腹体外实验模型,热CO2气腹(43℃,2～4h)作用于结肠癌细胞株COLO205细胞。以WST-8法检测细胞增殖,Annexin-V/PI流式细胞术及透射电镜检测细胞死亡,流式细胞术检测细胞周期。结果:热CO2气腹(43℃,2～4h)对结肠癌细胞有显著的增殖抑制作用,热CO2显著诱导细胞凋亡及G1期细胞阻滞。结论:热CO2气腹通过诱导细胞凋亡及G1期细胞周期阻滞显著抑制结肠癌细胞的增殖,热CO2气腹具有应用于结直肠癌腹膜种植转移治疗的潜能。

Objective To evaluate the anti-proliferative effect of hyperthermic CO2 pneumoperitoneum （HT-CO2） on colonic cancer cells, to define its mechanism of action, and to assess the feasibility as a new therapeutic procedure for peritoneal carcinomatosis in colon cancer. Methods Based on an in vitro HT-CO2 study model, the cell proliferation was determined with WST-8 assay after treatment by HT-CO2 at 43℃ for 24 hours. Apoptosis was assessed by Annexin-V/PI flow cytometry and transmission electron microscopy. Cell cycle was detected by PI flow cytometry. Results HT-CO2 produced in significant anti-proliferative effect on colonic cancer cells. HT-CO2 sensibly induced apoptosis and G1 phrase cell cycle arrest. Conclusions HT-CO2 can significantly inhibit the proliferation of colonic cancer cells, and the anti- proliferation is attributable to induction of apoptosis and G1 phrase cell cycle arrest. HT-CO2 may potentially serve as a therapeutic procedure for the management of peritoneal carcinomatosis caused by colon cancer.