增强子结合蛋白家族一个新基因的3′-非编码区序列对肝癌细胞7721生长的影响

3′ UNTRANSLATED REGION (3′ UTR) OF A NOVEL MEMBER OF C/EBP GENE FAMILY INFLUENCED THE GROWTH OF HEPATOCELLULAR CARCINOMA 7721 CELLS

目的观察所获得的增强子结合蛋白家族一个新基因ＨＰ８的３′┐非编码区（３′ＵＴＲ）对肝癌细胞生长的影响。方法将３′ＵＴＲ序列亚克隆至真核表达载体ｐＢＫＣＭＶ中，运用磷酸钙沉淀法转染肝癌细胞７７２１。结果观察到细胞的生长受到较明显抑制。结论表明该基因参与真核细胞转录调控，结合以前的实验结果（该基因在肝癌细胞中普遍高表达）。

Objective: Influence of 3′ UTR (untranslated region),a novel member of C/EBP gene family,on the growth of hepatocellular carcinoma 7721 cells was studied. Methods: The 3′ UTR was transfected in an eukaryotic expression vector pBKCMV into 7721 cells. Results: Transformation of 7721 cells was observed implicating that the cell growth could be inhibited. Conclusions: This gene could be a transactivator related to cell transcription,especially to cell growth.