摘要
目的观察所获得的增强子结合蛋白家族一个新基因HP8的3′┐非编码区(3′UTR)对肝癌细胞生长的影响。方法将3′UTR序列亚克隆至真核表达载体pBKCMV中,运用磷酸钙沉淀法转染肝癌细胞7721。结果观察到细胞的生长受到较明显抑制。结论表明该基因参与真核细胞转录调控,结合以前的实验结果(该基因在肝癌细胞中普遍高表达)。
Objective: Influence of 3′ UTR (untranslated region),a novel member of C/EBP gene family,on the growth of hepatocellular carcinoma 7721 cells was studied. Methods: The 3′ UTR was transfected in an eukaryotic expression vector pBKCMV into 7721 cells. Results: Transformation of 7721 cells was observed implicating that the cell growth could be inhibited. Conclusions: This gene could be a transactivator related to cell transcription,especially to cell growth.
出处
《肿瘤》
CAS
CSCD
北大核心
1997年第1期1-3,共3页
Tumor
基金
癌基因与相关基因国家重点实验室基金
关键词
肝肿瘤
基因
肿瘤细胞
细胞分化
Liver neoplasms Genes,C/EBP Cell differentiation Transfection Tumor cells,cultured