亚硒酸钠和维甲酸联合对HL-60细胞DNA与蛋白质合成和细胞周期的影响

COMBINED EFFECTS OF SODIUM SELENITE AND ALL TRANS RETINOIC ACID ON DNA AND PROTEIN SYNTHESES AND CELL CYCLE OF HUMAN PROMYELOCYTIC LEUKEMIA CELLS(HL 60)

目的观察亚硒酸钠和维甲酸联合作用对ＨＬ┐６０细胞ＤＮＡ与蛋白质合成和细胞周期的影响。方法以人早幼粒白血病细胞（ＨＬ┐６０）为实验对象，利用３Ｈ┐脱氧胸苷（３Ｈ┐ＴｄＲ）和３Ｈ┐亮氨酸（３Ｈ┐Ｌｅｕ）参入实验以及流式细胞仪进行分析。结果５．８μｍｏｌ／Ｌ亚硒酸钠和０．１μｍｏｌ／Ｌ维甲酸（ＲＡ）联合可显著抑制ＨＬ－６０细胞的ＤＮＡ和蛋白质合成，联合作用强于两者单独作用。流式细胞分析的结果表明：单独硒组的ＨＬ┐６０细胞在Ｇ２＋Ｍ期和Ｓ期有一定的阻滞；维甲酸的作用主要是阻滞Ｇ１期向Ｓ期移行，使Ｇ１期细胞蓄积，但该剂量单独作用较小；联合组主要表现在Ｇ１期阻滞作用要大于单独ＲＡ组的作用。

Objective:Combined effect of sodium selenite (Na 2SeO 3) and all trans retinoic acid (RA) on DNA and protein syntheses and cell cycle of HL 60 cell line was studied. Methods: Assays of 3H TdR and 3H Leu incorporation and the flow cytometric analysis were undertaken. Results: Either 5 8μmol/L Na 2SeO 3 or 0 1μmol/L RA decreased the syntheses of DNA and protein,but combination of two chemicals decreased more significantly. Na 2SeO 3 could slightly arrest HL 60 cells at G 2+M and S phases,RA increased G 1 and reduced S and G 2 cells,while Na 2SeO 3 plus RA increased G 1 more significantly Conclusions: Combined effect of Na 2SeO 3 and RA was much stronger than either of the two chemicals alone.