高效液相色谱法测定氯呋滴耳液的含量

Determination of Chloramphenicol Furacilin Ear Drops by HPLC

目的建立氯呋滴耳液含量测定的高效液相色谱法。方法色谱柱为Shim-pack VP-ODS C18;流动相为甲醇-水-三乙胺-冰醋酸（50∶50∶0.2∶1）;检测波长为240nm;柱温为室温;流速为1ml/min。结果呋喃西林在0.004 052-0.08104mg.ml^-1（r=0.999 9,n=6）,氯霉素在0.124 74-2.494 76mg.ml^-1（r=0.999 9,n=6）范围内呈良好线性关系;平均回收率分别为99.61%,98.82%;RSD分别为0.47%,0.98%（n=9）。结论本法检测快速,定量准确,可用于氯呋滴耳液的定量分析。

Aim To establish an HPLC method for determining the contents of Chloramphenicol Furacilin Ear drops. Methods The stationary phase was Shim-pack VP-ODS C18; the mobile phase was methanol-H2O-thiethylamine-Acetic acid giacial（50： 50： 0.2：1 ） ;the flow rate was 1mol·min^-1 and the column temperature was the room temperature. Results There was a good linear relationship when the content of furacilin and chloramphenicol was within the range of 0. 004 052 - 0.081 04mg·ml^ - 1 （ r = 0. 999 9, n = 6） and 0.124 74 - 2. 494 76mg·ml ^- 1 （ r = 0.999 9, n =6） respectively. The average recovery rate was 99.61%, （ n =9, RSD =0.47% ） and 98.82%, （ n = 9, RSD = 0.98% ）. Conclusions This method is quick and accurate. It can be used for quality control of Chloramphenicol Furacilin Ear Drops.