高效液相色谱法测定附桂骨痛胶囊中淫羊藿苷的含量

Determination of Icariin in Fuguigutong Capsule by HPLC

目的建立HPLC方法测定附桂骨痛胶囊中淫羊藿苷的含量。方法色谱柱：Shim-pack VP-ODS（150mm×4.6mm I.D）;流动相：乙腈-水（25∶75）;流速为1.0ml/min;检测波长为270nm;柱温：室温。结果淫羊藿苷在20-160ng.ml-1（r=0.999 6,n=9）范围内与峰面积呈良好的线性关系,平均回收率为100.4%,RSD为1.2%（n=6）。结论本法分离度好,快速,简便。可用于本制剂中淫羊藿苷的测定。

Aim To establish a method for determination of icariin in Fuguigutong Capsule by HPLC. Methods The Shlm-pack VP-ODS（ 150mm × 4.6mmI. D ） was adopted at room temperature. The mobile phase was acetonitrile-water （25：75 ） . The flow rate was 1.0ml/min and the detection wavelength was 270nm. Results The linear responses ranged from20 - 160ng·ml^-1 of icariin （ r = 0. 999 6, n = 5 ）. The average recovery of terantrine was 100.4% , RSD = 1.2% （ n = 9）. Conclusions The method is simple, accurate , and suitable for the determination of icariin in Compound Fuguigutong Capsules.