慢性阻塞性肺疾病中氧化应激的研究

The study of oxidative stress in patients with Chronic obstructive Pulmonary disease

目的:通过测定慢性阻塞性肺疾病(COPD)患者和正常对照组血清中8-差向前列腺素的水平,探讨COPD患者中氧化应激的水平。方法:20例急性加重期和缓解期的COPD患者、16例吸烟对照和24例不吸烟对照,COPD急性加重期和缓解稳定期、正常对照组分别采集外周静脉血离心,血清中游离8-差向前列腺素的测定采用酶分析法(EIA)。结果:COPD患者主要肺功能FEV1%和FEV1/FVC(分别为50.1±17.27和56.2±10.31)与对照组(分别为106.0±20.8和83.4±6.34)比较明显降低,结果差异有统计学意义(P<0.001)COPD急性加重期血清中游离8-差向前列腺素的浓度(5.42±2.416ng/ml)明显高于稳定期的浓度(3.30±2.267ng/ml),差异有统计学意义(P<0.01);吸烟对照组COPD稳定期血清中游离8-差向前列腺素的浓度(5.72±2.195ng/ml)高于COPD稳定期(3.30±2.267ng/ml)和不吸烟对照组(4.30±1.964ng/ml)的浓度,结果差异有统计学意义(P<0.05)。COPD血清中游离8-差向前列腺素和FEV1%、FEV1/FVC无显著相关性(r分别为-0.076和-0.046,P>0.05)。结论:在急性加重期的COPD患者和健康吸烟者,存在氧化应激水平的明显升高。COPD患者中氧化应激负荷可能和气流阻塞无相关。

Objective： To investigate the oxidative stress burden in patients with chronic obstructive pulmonary disease （COPD）. Methods： Serum samples of 20 patients with COPD in severe exacerbation and stable stage, 16 healthy smokers and 24 healthy non - smokers were collected for measurements of the level of free 8 - isoprostane. The free 8 - isoprostane in serum was measured by enzyme immunoassay（EIA）. Results： Significantly decreased FEV1% AND FEVI/FVC were shown in COPD patients,as compared to the healthy control group[ （50.1 ± 17.27）vs. （106.0± 20.82）,P〈 0.01 and （56.2 ± 10.31）vs. （83.4 ± 6.34）,P〈 0.01 ,respectively]. In severe exacerbation of COPD,the level of free 8 - isoprostane was significantly higher than stable stage of COPD（5.42 ± 2.416ng/ml, 3.30 ± 2. 267ng/ml, respectively, P 〈 0.01 ）. In healthy smokers, the level of free 8 - isorostanein serum was higher than healthy non- smokers[ （5.72±2. 195）ng/ml, （4.3 ± 1.964） ng/ml, respectively, P〈 0.05]. The level of free 8- isoprostane in serum had nocorrelation with FEVI % and FEVI/FVC in patients with COPD（ r = -0.076 and -0.046, respectively, P 〉 0.05 ）. Conclusion： Increased systemic oxidative stress occurs in patients with severe exacerbation of COPD and in the healthy smokers. The oxidative stress burden may not correlate significantly to the obstruction of airways.