TNF-α在IVF-ET后自然流产患者绒毛及蜕膜组织中的表达

Expression of Tumor Necrosis Factor-α in Villi and Decidua Tissues of Spontaneous Abortion Following IVF-ET

目的:探讨肿瘤坏死因子-α(TNF-α)在胚胎早期发育过程中的作用及其与流产的关系。方法:应用免疫组织化学标记法(SP法),比较IVF-ET后自然流产(10例,A组)、复发性流产(20例,B组)、人工流产(20例,C组)3组患者早孕绒毛、蜕膜组织中TNF-α的表达情况。结果:TNF-α在3组绒毛及蜕膜组织中均有表达,A、B组蜕膜及绒毛组织中表达的平均光密度与C组相比差异有非常显著性(P<0.01)。结论:TNF-α调节生殖免疫过程,参与了母-胎间相互作用,TNF-α表达异常,在自然流产发病中起重要作用,是导致自然流产的原因之一。

To compare the expressions of TNF-α in villi and clecidua tissues of spontaneous abortion after IVF-ET or natural conception and of artificial abortion, and to understand its effect on early embryo development. Methods： Using immunohistochemical technique （SP methods）, the expressions of TNF-α were tested in villi and decidua tissues from spontaneous abortion, 10 cases after IVF-ET （IVF-ET group） and 20 cases from natural conception （ spontaneous group）, and from 20 cases of artificial abortion of normal pregnancy （ normal group）. Results：TNF-α expressed in all early pregnancy tissues. Compared to the normal group, TNF-α expression in both spontaneous abortion groups had significant difference （ P〈 0.01 ） .Conclusions：This study further confirms that TNF-α adjust reproductive immunity and effect matemal-fetal interface. The abnormal expressions of TNF- α play an important role in spontaneous abortion which should be one of reasons.