高效液相色谱法测定肾舒颗粒中盐酸小檗碱的含量

Determination the Content of Berberine Hydrochloride in Shenshu granules by HPLC

目的:建立以高效液相色谱法测定肾舒颗粒中盐酸小檗碱含量的方法。方法:色谱柱:资生堂ODS C_(18),流动相:乙腈-磷酸二氢钠(0.025 mol·L^(-1))-十二烷基硫酸钠(0.025 mol·L^(-1))(50:25:25),柱温:25℃,检测波长:365 nm,流速:1.0 ml·min^(-1)。结果:盐酸小檗碱进样量在0.19～1.9μg范围内线性良好(r=0.999 9),平均回收率:99.34%,RSD 1.76%(n= 6)。结论:本方法简便,准确、重复性好,可用于该制剂的质量控制。

Objective： To establish an HPLC method for determination the content of berberine hydrochloride in Shenshu granules. Method： The SHISEIDO ODS C18 column was used;mobile phase was acetonitrile-sodium dihydrogen phosphate （0. 025 mol·L^-1）-sodium dodecyl sulfate（0. 025 mol·L^-1） （50： 25：25 ） ; column temperature was 25 ℃ ;detection wavelength was at 365 nm; flow rate was 1.0 ml·min^-1. Result： The calibration curve of berberine hydrochloride had a good linear relationship in the range of 0.19 - 1.9 μg （ r = 0.999 9 ） , the average recovery was 99.34%, the RSD was 1.76% （ n = 6 ）. Conclusion： The method is simple, accurate and reproductive. It can be used for quality control of this preparation.