HPLC法测定复方甘草酸苷胶囊中三组分的含量

Determination the Contents of Three Components Compound Glycyrrhizin Capsules by HPLC

目的:采用HPLC法测定复方甘草酸苷胶囊3组分的含量。方法:甘草酸苷:用ODS-C_(18)柱(150 mm×4.6 mm,5μm);2%冰醋酸-乙腈(65:35)为流动相,流速为1.0 ml·min^(-1),检测波长为252 nm;甘氨酸和蛋白酸:采用2,4-硝基氟苯柱前衍生化方法,色谱柱ODS C_(18)(250 mm×4.6 mm,5μm);乙腈-0.05 mol·L^(-1)醋酸钠缓冲液(35:65),检测波长:360 nm,流速1.0 ml·min^(-1)。结果:甘草酸苷、甘氨酸和蛋氨酸的线性范围分别为103.3～826.7μg·ml^(-1)(r=0.999 8),6.25～50.0μg·ml^(-1) (r=0.999 5)和6.33～50.67μg·ml^(-1)(r=0.999 5),其回收率分别为99.8%,99.7%和99.8%,RSD分别为0.3%,0.3%和0.4 (n=9)。结论:该法简便、灵敏、准确。

Objective： To establish an HPLC method for determination the contents of glycyrrhizin, glycine and methionine in compound glycyrrhizine capsules. Method： Glycyrrhizine：Kromosil C18 ODS column（ 150 mm × 4.6 mm,5 μm） was used, 2% glacial acetic acid-Acetonitrils （35：65）as mobile phase, the flow rate was 1.0 ml·min^-1. The detection wavelength was 252 nm. The glycine and methionine were measured by the C18 reversed phase HPLC with 2,4-Dinitro-fluorobencene of precolumn derivatication. The Da you C18 column （250 mm × 4.6 mm,5 μm） was used, the acetonitrile-0.05 mol·L^-1 sodium acetate Buffer solution （ 35： 65） as mobile phase, the flow rate was 1.0 ml·min^-1, the detection wave length was 360 rim. Result： The linear ranges of glycyrrhizin, glycine and methionine were at 103.3 -826.7 μg·ml^-1 （r =0.999 8）, 6.25 -50.0 μg·ml^-1 （r =0.999 5） and 6.33 -50.67 μg·ml^-1 （r = 0.999 5 ）, respectively, the average recoveries （ n = 9） were 99.8% ,99.7% and 99.8% with RSD 0.3%, 0.3% and 0.4%, respectively. Conclusion： The method is convenient, sensitive and accurate.