封堵器透明质酸钠肝素微囊化涂层的研制

The Preparation of Sodium Hyaluronate Heparin Microencapsulated Coating in Occluder

目的本研究将封堵器进行肝素涂层,通过局部肝素的缓慢释放,达到抗血栓目的,避免先心病介入治疗后需要长期服用抗血栓药物来避免血栓形成的现状。方法首先将肝素降解成低分子肝素,借助油包水(w/o)乳化交联技术制备了新型肝素微胶囊,并对其性能进行了一定的研究。同时,将制备的肝素明胶粒子采用透明质酸钠涂层方法进行镍钛合金封堵器的涂层。结果各种影响因素中明胶浓度对肝素微胶囊制备影响最大,明胶浓度为1%时肝素微胶囊粒径多在100 um以内,最佳制备工艺所得LMWH-GMS的包封率约为80%;封堵器采用透明质酸钠涂层时涂层均匀、不易脱落。结论本研究成功地将肝素降解成低分子肝素,通过微胶囊化技术可将低分子肝素制成粒径纳米级微胶囊并保持抗血栓特性;应用透明质酸钠涂层方法可以成功地对镍钛合金封堵器进行涂层。

Objective NiTi alloys oceluder was coated with heparin in this study. Release heparin slowly for anticongulation. And eating anticongulation drugs avoids congulation after transcatheter closure of congenital heart disease. Methods First heparin was degraded into low molecular weight heparin, then, by using the gelatin as microcapsule materials,we prepared new heparin microcapsule through the cross-linking technology for water-in-oil（w/o） emulsification and did some studies to the performance. At the same time, heparin microcapsule was coated NiTi alloys respectively by glutin and sodium hyaluronate. Results The research showed that concentration of gelatin played an major role in preparation of mierocapsule. When concentration of gelatin was 1% ,the diameter was about 100 urn. The envelope efficiency of heparin microcapsule was about 80% with the best technique. The coating above NiTi alloys occluder coated with sodium hyaluronate was well-distributed and not easy to drop. Conclusion Low molecular weight heparin can be successfully produced by heparin in this study. Nanometer microcapsule was made from low molecular weight heparin by microcapsule technique, which may maintain the characteristics of antithrombus. Nanometer microcapsule was made from low molecular weight heparin by microcapsule technique, which may maintain the characteristics of antithrombus. And NiTi alloys occluder can be successfully coated with heparin microcapsule by using sodium hyaluronate.