超滤法提取茶多糖研究

以截留分子量为30000D的超滤膜对茶叶酶解提取液进行超滤分离,考察了操作压力、运行时间对膜渗透通量的影响;然后对由超滤截留液醇沉制取的粗多糖进行精制,考察了三氯乙酸浓度对精制过程的影响。结果表明,采用超滤法可以有效实现茶叶酶解提取液中大分子多糖与小分子多酚等活性物质的分离,三氯乙酸浓度为4%时,多糖得率34.3%,含糖量52%。

Tea leaves enzymolysis extracting solution was separated with ultrafiltration membrane which molecular weight is 30000D. The effects of the operation pressure and running time on the membrane permeation flux were studied and discussed briefly. And then ultrafiltration trapped fluid was purified. The effect of trichloroacetic acid concn on purification processes was studied. The results showed that tea leaves enzymolysis extracting solution could be separated with ultrafiltration, tea polysaccharide final yield is 34.3%, sugar content is 52% when trichloroacetic acid concn is 4%.