鸡腿蘑深层发酵条件的优化

以生物量和胞外多糖含量为测定指标,通过摇瓶培养的方式对鸡腿蘑发酵条件的优化进行了研究。结果表明:鸡腿蘑摇瓶发酵条件为起始pH6.0,转速170 r/m in,装量系数为0.4,接种量10%,发酵时间为6d,此时生物量可达17.70g/L,胞外多糖含量达1.46g/L。

Using biomass and content of extracellular polysaccharide as indexes, optimization of fermentation condition was studied by shaking culture. The results showed that the suitable shaking culture condition was as follows： initial pH of medium 6.0, shaking frequency 170r/min, 100mL liquid medium in size 250mL shaking flask, inoculation quantity 10%, fermentative time 6 days. Under this condition, the production of 17.70g/L biomass and 1.46g/L extracellular polysaccharide were obtained.