马缨杜鹃继代培养培养基配方研究

Study on Subculture Medium Prescription of Rhododendron delavayi Franch

[目的]研究马缨杜鹃组织培养中继代培养阶段的培养基,以期筛选出最适继代培养培养基配方,为最终建立马缨杜鹃无毒快繁体系奠定基础。[方法]以1/4 MS培养基为基本培养基,附加不同浓度的2-ip和6-BA,并以食用白砂糖代替分析纯蔗糖,对马缨杜鹃进行继代培养,比较芽诱导率和生长状况。[结果]试验结果表明,2-ip能促进马缨杜鹃单芽茎段增殖,6-BA不能;当2-ip浓度为1.50mg/L时,增殖系数和平均株高均高,分别为2.10和3.60 cm。[结论]最适继代培养基为1/4 MS,30 mg/L蔗糖,8 mg/L琼脂,2-ip 1.50 mg/L,NAA1.50 mg/L,可以用食用白砂糖代替分析纯蔗糖对腋芽的诱导。

[Objective] Subculture medium of Rhododendron delavayi Franch was studied to select the optimal subculture medium prescription and to establish the viral-free rapid propagation system finally. [Method] 2-ip and 6-BA were supplemented in 1/4MS basal medium with edible sugar taking the place of sucrose of analytical pure. Rhododendron delavayi was subcuhured and bud induction rate and growth status were compared. [Conclusion] The optimum subculture medium was 1/4MS ＋ 2-ip 1.50 mg/L＋NAA 1.50 mg/L＋30mg/L sucrose＋8 mg/L agar. Edible sugar could take place of analytic sucrose to induct axillary bud.