摘要
[目的]明确不同加硒量对蛹虫草主要活性成分含量的影响。[方法]向固体培养基中加入不同含量的亚硒酸钠,接种培养后用高效液相色谱法测定虫草素和腺苷,用高碘酸钠法测定虫草酸,用苯酚-硫酸法测定虫草多糖。[结果]虫草素在加硒量为5.5mg/kg时达到4.50mg/g,虫草酸在加硒量为4.0mg/kg时达到30.06mg/g,腺苷和虫草多糖含量与加硒量无明显相关性。[结论]加硒可显著提高蛹虫草固体培养物中虫草素和虫草酸含量,但医疗保健功效的提高还需通过动物试验加以验证。
[Objective] The aim of this paper was to acquire the effect of different adding amount of selenium on main active component contents of Cordyceps militaris (CM). [Method] Different contents of Na2SeO3 were added into the solid substrate.After inoculation, HPLC method was used for determination of cordycepins and adenosines. Sodium periodate method and phenol-sulphuric acid method were used for the determination of mannitols and polysaccharides. [Result] Content of cordycepins attained 4.50 mg/g when 5,5 mg/kg selenium was added. Mannitols content reached 30.06 mg/g at selenium being 4.0 mg/kg. Adenosines and polysaccharides had no obvious relativity with the amount of selenium. [Conclusion] Contents of cordycepins and mannitols of CM could be advanced by adding selenium. But the function of medical treatment and health care needed to be verified by animal experiment.
出处
《安徽农业科学》
CAS
北大核心
2007年第29期9293-9294,共2页
Journal of Anhui Agricultural Sciences
关键词
蛹虫草
亚硒酸钠
虫草素
腺苷
虫草多糖
虫草酸
Cordyceps militaris
Na2SeO3
Cordycepins
Adenosines
Cordyceps polysaccharides
Mannitols