加硒对蛹虫草主要活性成分含量的影响

Effect of Adding Selenium on Main Active Component Contents of Cordyceps militaris

[目的]明确不同加硒量对蛹虫草主要活性成分含量的影响。[方法]向固体培养基中加入不同含量的亚硒酸钠,接种培养后用高效液相色谱法测定虫草素和腺苷,用高碘酸钠法测定虫草酸,用苯酚-硫酸法测定虫草多糖。[结果]虫草素在加硒量为5.5mg/kg时达到4.50mg/g,虫草酸在加硒量为4.0mg/kg时达到30.06mg/g,腺苷和虫草多糖含量与加硒量无明显相关性。[结论]加硒可显著提高蛹虫草固体培养物中虫草素和虫草酸含量,但医疗保健功效的提高还需通过动物试验加以验证。

[Objective] The aim of this paper was to acquire the effect of different adding amount of selenium on main active component contents of Cordyceps militaris （CM）. [Method] Different contents of Na2SeO3 were added into the solid substrate.After inoculation, HPLC method was used for determination of cordycepins and adenosines. Sodium periodate method and phenol-sulphuric acid method were used for the determination of mannitols and polysaccharides. [Result] Content of cordycepins attained 4.50 mg/g when 5,5 mg/kg selenium was added. Mannitols content reached 30.06 mg/g at selenium being 4.0 mg/kg. Adenosines and polysaccharides had no obvious relativity with the amount of selenium. [Conclusion] Contents of cordycepins and mannitols of CM could be advanced by adding selenium. But the function of medical treatment and health care needed to be verified by animal experiment.