摘要
目的:建立高效液相色谱法测定苦参素胶囊中氧化苦参碱的含量和有关物质的量。方法:采用 C_(18)色谱柱(250 mm×4.0 mm,5μm);以0.05 mol·L^(-1)磷酸二氢钾溶液(用磷酸调 pH 至3.5)-甲醇(83:17)为流动相,流速:1.0 mL·min^(-1),检测波长215 nm。结果:在建立的色谱条件下,氧化苦参碱与杂质能完全分离。氧化苦参碱线性范围为40.0~200μg·mL^(-1)(r=0.9999)。结论:所用方法简便、准确,专属性好。
Objective: To establish an RP - HPLC method for the determination of content and related substances of marine capsules. Methods: C 18 column (250 mm × 4. 0 mm,5 μm) was used. The mobile phase consisted of potassium dihydrogen phosphate solution( adjust to pH 3.5 with phosphoric acid) -methanol (83:17 ). The UV detective wavelength was 215 nm. Results:Under the described chromatographic condition oxymatrine was completely separated from its impurities. The linear range for the content of oxymatrine was 40. 0 - 200 μg · mL^ -1 ( r = 0. 9999). Conclusion :The method is simple, rapid and accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第9期1441-1443,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家重点新产品项目(2002ED880005)
科技部农业科技成果转化资金项目(2005640120279)