丝裂原活化蛋白激酶信号通道对大鼠创伤性深静脉血栓形成影响的实验研究

Study the influence of MAPK signaling pathway in a rat model of traumatic deep vein thrombosis

目的探讨丝裂原活化蛋白激酶(MAPK)信号通路对创伤性深静脉血栓(TDVT)形成的意义。方法将150只SD大鼠随机分为正常对照组(A,10只)和模型组(140只)。模型组根据造模后的不同生物学状态再分为七组:创伤即刻组(B)、血栓形成前期组(C)、高峰期血栓形成组(D)、高峰期血栓不形成组(H)、血栓消退期组(E)、血栓不消退组(F)和血栓不形成组(G),在相应时相点无创切取股静脉血管组织,随后抽取各组大鼠总RNA,用Genechip Rat Genome 430 2.0芯片测定股静脉RNA表达,并分析MAPK信号通路基因表达变化情况。结果MAPK通路中FGF、TGFB、G12、ERK、MKPPAK1/2、MEKK2/3、AKT、JNK、FLNA、NUR77、C-JUN、ATF2、P53、MAX等关键基因均呈下调,调控着细胞的增殖、分化、炎症、凋亡、抗凋亡、细胞周期。结论MAPK信号通路可能是调控血栓的生物学状态的重要信号通路之一。

Objective TO study the significance of MAPK signaling pathway in traumatic deep vein thrombosis. Method 150 rats were divided into control （ group A ） and experience groups. The experience group were divided into 7 groups according to biological states ： the post - traumatic instant （ group B ）, the initial period of thrombosis （ group C ）, the crest - time of thrombosis （ group D ）, without thrombosis at the crest - time （ group H ）, thrombi solution （ group E ）, thrombi insolution （ group F ） and no thrombosis（group G）groups. To incise the femoral vein in corresponding time, to adopt to Trizol onestep method for total RNA extraction of femoral vein. After applying Genechip Rat Genome 430 2.0 genechips to detect the RNAs expressions, the genes related to MAPK signaling pathway were selected through genechip data analyasis. Result The key genes related to MAPK signaling pathway of FGF, TGFB, G12, ERK, MKPPAK1/2, MEKK2/3, AKT, JNK, FLNA, NUR77, C - JUN, ATF2, P53, MAX were down regulation, which controled the proliferation, differentiation ;inflammation; apoptosis, anti - apoptosis and cell cycle of the vascular endothelial cell. Conclusion MAPK Signaling Pathway may be one of the most important signaling pathway to control the biological states of thrombi.