雷公藤复杂提取物脂质体的制备及稳定性研究

Preparation of liposomes containing extracts of Tripterygium wilfordii and evaluation of its stability

目的：对雷公藤复杂提取物进行脂质体包合,探讨脂质体包合复杂物质的可行性。方法：采用薄膜-超声法制备脂质体,考察工艺条件以及处方组成对主要成分包封率的影响以及脂质体储存过程中的稳定性。结果：薄膜-超声法制备的脂质体为小单室脂质体,粒径在30-50 nm,主要成分雷公藤红素及雷公藤生物总碱的最大包封率分别为98.10%,88.63%,但是包封有复杂物质的脂质体混悬液长时间存放不稳定。结论：脂质体可以用于包合复杂混合物,但长期存放呈现一定的不稳定性。

Objective：The liposomes containing extracts of Tripterygium wilfordii were prepared and the possibility of entrapment of complex chemicals by liposomes were studied.Method： The liposomes containing extracts of T.wilfordii were prepared by thin-film dispersion method,the effect of process parameters and composition of materials on the entrapment efficiency of the main components were studied.The stability of the liposomes dispersion was also evaluated.Result： The liposomes made by thin-film dispersion method were mostly small unilamellar vesicles and their particle size was 30 nm～50 nm.The optimum entrapment efficiency of tripterine and the total alkaloids were respectively 98.10% and 88.63% but the liposomes dispersion was unstable when kept at 4 ℃.Conclusion： The complex chemicals can be entrapped by the liposomes,but its stability need to be improved furtherly.