RP-HPLC法同时测定金银花中的2种三萜皂苷

Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC

目的：建立同时测定金银花中三萜皂苷成分忍冬苦苷A和忍冬苦苷C含量的方法。方法：采用反相高效液相色谱法,A lltech C18柱（4.6 mm×250 mm,5μm）,流动相甲醇-乙腈-0.1%磷酸,检测波长210 nm。结果：忍冬苦苷A和忍冬苦苷C分别在0.15-2.25μg（r=0.999 9）和0.11-1.65μg（r=0.999 1）,呈现良好线性关系,平均加样回收率分别为99.9%,98.3%。结论：忍冬苦苷A和忍冬苦苷C在不同地区的金银花药材中含量差异较大。

Objective：To develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae.Method： The analysis was carried out on an Alltech C18 column（4.6 mm×250 mm,5 μm） evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase.Flow rate was at 1.0 mL·min^-1 and the detection wavelength was at 210 nm for UV detection.Result： The calibration curves were linear over the range from 0.15 to 2.25 μg（r=0.999 9） for loniceroside A and 0.11 to 1.65 μg（r=0.999 1） for loniceroside C.The average recoveries were 99.9% and 98.3%,respectively.Conclusion： The contents of Loniceroside A＆C are diverse in Flos Lonicerae japonicae in different regions.