戊己丸提取物不同配伍方对豚鼠离体结肠运动影响的实验研究

Experimental studies of effects of Wujiwan extracts in different compatibilities on motility of isolated colon in guinea pig

目的:观察戊己丸提取物优选方对不同状态豚鼠离体结肠的影响,同时比较2种配伍比例作用的异同点。方法:通过乙酰胆碱和肾上腺素分别诱导豚鼠离体结肠运动亢进和抑制状态,在反应体系中分别滴加戊己丸1号方、2号方,观察其对豚鼠不同状态离体肠管的影响,同时用B liss法计算其半数有效浓度(EC50)。结果:对于离体结肠运动亢进模型,戊己丸1号方、2号方均可抑制结肠运动亢进,在本试验观察的时间内有明显的时间依赖性和浓度依赖性,且1号方EC50在设计观察的各时间段内均低于2号方;对于抑制模型,1号方300 mg.L-1,在15 m in时有拮抗作用(P<0.05),2号方30 mg.L-1,在9,15 m in时与模型组相比有差别(P<0.05)。结论:戊己丸1号方、2号方可显著抑制乙酰胆碱所致的结肠运动亢进;1号方、2号方对肾上腺素所致结肠运动抑制还有一定的作用;随配伍比例的不同,二者在共同药效作用的基础上,呈现出不同的作用特点和优势。

Objective：To investigate the effects of Wujiwan extracts in two different compatibility proportions（named the No.1 and No.2 respectively） on motility of isolated colon in guinea pig,and compare the therapeutic effects with each other.Method： To observe the function of the No.1 and No.2 with different concentrations on colon contraction induced by acetylcholine（Ach） and inhibition induced by adrenalin（Ad） respectively,and calculate the EC50 with the method of Bliss.Result： The No.1 and No.2 both could significantly inhibit the contraction of colon,and in 15 minutes,there was significant time-dependence and concentration-dependence,and EC50 of the No.1 was lower than that of No.2.However,but when the colonic was inhibited by Ad,at the time of 9 min and 15 min,the No.1 at the level of 300 mg·L^-1,and at the time of 15 min,the No.2 at the level of 30 mg·L^-1,could both excite the colon significantly（P〈0.05）.Conclusion： Our experiments indicate that the No.1 and No.2 both can significantly inhibit the colon contraction in guinea pig,and the No.1 is superior to the No.2 to some extent.And at the same time,we also can conclude that the No.1 and No.2 have exciting effects on inhibitory colon.Meanwhile,the No.1 and No.2 each have its own advantages based on the common therapeutic effects.