地黄水提液对2型糖尿病大鼠胰岛素抵抗及resistin基因mRNA和蛋白表达的影响

Effect of Rehmannia glutinosa water extraction on insulin resistance and gene expression of resistin in type 2 diabetes mellitus rats

目的:探讨中药地黄水提液对2型糖尿病大鼠糖脂代谢和resistin基因表达的影响。方法:Wistar大鼠喂以高热量饲料加链脲佐菌素建立2型糖尿病大鼠模型,随机分为正常对照组、2型糖尿病模型组、地黄高、中、低剂量治疗组。8周后,以RT-PCR法检测大网膜脂肪组织resistin基因mRNA的表达,以SDS-PAGE检测其蛋白的表达。同时观察空腹血糖、空腹血胰岛素、血脂的含量和胰岛素抵抗指数的变化,研究不同剂量地黄水提液对上述各指标的影响。结果:地黄治疗组大鼠较模型组大鼠resistin基因mRNA和蛋白表达显著降低(P<0.05)。各治疗组FPG,FINS,TG,LDL,CH的含量及IR显著降低(P<0.05),而HDL则显著升高(P<0.05)。结论:地黄水提液通过抑制脂肪组织抵抗素基因的表达,降低血胰岛素抵抗水平、改善脂代谢紊乱,从而降低2型糖尿病大鼠血糖。

Objective：To investigate the effect of Rehmannia glutinosa water extraction（RGL） on adipose metabolic disorder and gene expression of resistin in type 2 diabetes mellitus rats.Method： The wistar rats model of 2-DM were induced by high calorie feeding and small dose injection of STZ.Rats were randomly divided into diabetic model group,diabetic model treated with RGL（2.4 g·kg^-1·d^-1）,RGL（1.2 g·kg^-1·d^-1）,RGL（0.6 g·kg^-1·d^-1） and normal control group.The levels of FPG,FINS,TG,HDL,LDL,CH and IR were measured,and the mRNA expression of resistin was determined by RT-PCR,the protein expression measured by SDS-PAGD at the end of 8 weeks.Result： The gene expression of resistin in RGL group were lower than that of diabetic model （P〈0.01）.The levels of FPG,FINS,TG,LDL,CH,IR in RGL group were lower than that of diabetic model（P〈0.05）,and HDL were higher（P〈0.05）.Conclude： RGL can improve insulin resistance in the experimental 2-DM rats,can effectively ameliorate adipose metabolic disturbance and decline IR and FINS by increasing the gene expression of resistin.