儿童慢性乙肝病毒携带者T细胞亚群与HBeAg的关系

Relationship between T-cell subsets and HBeAg in chronic hepatitis B-virus children carriers

目的研究儿童慢性HBV携带者的T细胞亚群状态。方法设正常对照组(n=32)及儿童慢性HBV携带(ASC)组(n=104),ASC组中又根据HBeAg分为HBeAg阳性(n=56)及阴性组(n=48)。血清HBV标志物经酶联免疫吸附试验(ELISA),T细胞亚群采用碱性磷酸酶-抗碱性磷酸(APAAP)免疫组化染色法检测。结果ASC组CD3+、CD4+、CD4+/CD8+明显低于对照组,CD8+明显高于对照组(P<0.01);其中HBeAg阳性组的CD4+、CD4+/CD8+较HBeAg阴性组明显升高(P均<0.01或0.05);CD8+明显降低(P<0.01)。结论儿童慢性HBV携带者存在T细胞亚群功能紊乱。其中,尤以HBeAg阳性者为著。

Objective To investigate the relationship between the T - cell subsets and HBeAg in chronic hepatitis B - virus carrier children. Methods One handred and four chronic hepatitis B - virus carrier children and 32 healthy children were selected , the former were divided into HBeAg（ ＋ ） group （ n =56） or HBeAg（ - ） group （ n =48） according to HBeAg. T - cell subsets in the peripheral blood were measured by APAAP, and HBV - signs were measured by ELISA. Results The mean concentrations of CD3 ＋ , CD4 ＋ , CD4 ＋ / CD8 ＋ of HBV - cartier children were significantly lower than those in control group （ P 〈 0. 01 ） . The level of CD8 ＋ was markedly higher （ P 〈 0.01 ）. Of them, CD4 ＋, CD4 ＋ / CD8 ＋ of HBeAg（ ＋ ） group were significantly higher than those of HBeAg（ - ） group and CD8 ＋ was markedly higher （ P 〈 0.01 ）. Conclusion There were deficiency of T - cell subsets in HBV - carrier children ,this deficiency were more obvious in HBeAg（ ＋ ） group