肝细胞癌组织中差异表达基因的初步筛选

Screening the Human Hepatocellular Carcinoma Associated Gene by Gene Chip

目的探讨基因表达谱芯片技术在筛查肝细胞癌相关基因群表达中的作用。方法采用美国Affymetrix公司的U133A2.0基因表达谱芯片,按一步法抽提肝细胞癌及正常肝脏组织总RNA,分离纯化两种组织的mRNA;经逆转录合成掺入生物素标记的cDNA合成探针,与芯片杂交和严格洗片后,用荧光扫描仪扫描芯片荧光信号图像,分析肝细胞癌及正常肝组织中差异表达的基因。结果在14500条基因中,肝细胞癌组织与正常肝脏组织间有2756条(19.01%)存在差异表达的基因,其中上调表达基因1772条和下调表达基因984条,对2756条差异表达基因作了初步功能分类,这些基因与肝细胞癌的发病机制存在相关性。结论基因表达谱芯片技术可以筛选出肝细胞癌表达异常的相关基因群,对其进一步研究有助于认识肝细胞癌的发病机制。

Objective To search for effectiveness of screening same differentially expressed genes of hepatocellular carcinoma（HCC） by GeneChip. Methods The total RNAs were isolated from tissues of two HCC and one normal liver by TRI-ZOL method and then purified,reversely transcribed to cDNA with incorporating biotin labeling probe,hybridizated with GeneChip（covering 18 400 transcripts, representing 14 500 distinct genes）, picture signals of fluorescence in gene array were scanned and compared differencial expression of gene in two tissues by computer analysis. Results Same differentially expressed genes were 2 756（19.01 %） and same up regulated and downregulated genes were 1 772 and 984 respectively in two HCC and one normal liver. These genes were classified initially according to function and played important role in pathogenesy of HCC by further analysis of the obtained genes. Conclusion The analysis of gene expression profile of HCC based on GeneChip can rapidly select associated abnormal genes ,further analysis of the obtained genes can help to illuminate internal pathogenesy of HCC.