细胞周期素D1和β-连环蛋白表达与胃癌的关系

Expression of β-catenin and CyclinD1 in Gastric Carcinoma

目的:探讨细胞周期素D1(Cyclin D1)和β-连环蛋白(β-cat)在胃癌表达及其与肿瘤生物学行为的关系。方法:应用免疫组化法检测50例胃癌组织、10例正常胃组织、20例胃腺瘤标本中细胞周期素D1和β-连环蛋白在胃癌中的表达。结果:胃癌β-cat的异常表达率(78.0%)高于胃腺瘤(60.0%),β-cat的表达异常与胃癌的分化程度、淋巴结转移密切相关,与胃癌的浸润深度、肿瘤大小无相关性。胃癌Cyclin D1的异常表达率(72.0%)高于胃腺瘤(60.0%),Cyclin D1的表达异常与胃癌的分化程度相关,而与胃癌的浸润、转移无相关性。结论:β-cat的异常表达与胃癌的分化和转移密切相关,β-cat异常表达导致Cyclin D1的激活而引起细胞增殖和分化失控。β-cat和Cyclin D1可作为反映胃癌生物学行为的指标。

Objective： To analyze the expression of CycinD1 and β -catenin in the tissues of gastric carcinoma and its lymphatic metastasis, and to explore their relationship, role in tumoral differentiation, invasion and metastasis. Methods： The expressions of CycinD 1 and β -catenin were detected by immunohistochemistry（IHC） in 50 cases of gastric carcinoma. Results.. Immunohistochemical analysis of tissue microarrays demonstrated β-catenin prolein expression in 78.0% of the gastric carcinoma and only in 60.0% of gastric adenoma. β-catenin expression was associated with tumor differrentiation status and lymph node metastasis. While in tumors depth and size, there was no significance. CycinD 1 expression was associated with tumor differrentiation status. While in tumors depth and lymph node metastasis, there was no significance. Conclusion： β -catenin expression was associated with invasion and metastasis of gastric carcinoma. The abnormal expression of β-cat activaes CycinD1, and Results in unchecked proliferation and differentiation of the cell, and these might be important factors for judging invasion of eastric carcinoma.