健脾清肠法治疗溃疡性结肠炎的临床疗效及对血清白细胞介素4和白细胞介素8的影响

Clinical Effects and Influence of Jianpiqingchang recipe on interleukin-4 and interleukin-8 expression in serum of ulcerative colitis patients

[目的]探讨健脾清肠法治疗溃疡性结肠炎(UC)患者的临床疗效及对血清白细胞介素4(IL-4)I、L-8的影响。[方法]72例UC患者随机分为2组,治疗组和对照组各36例,治疗组口服中药健脾灵片加苦参槐花合剂保留灌肠治疗,对照组单纯口服艾迪莎治疗。采用双抗体夹心ELISA法测定2组患者治疗前后血清IL-4I、L-8水平,並与30例健康志愿者(志愿组)对照。[结果]治疗组临床治愈率为66.7%,显效率为91.7%,对照组分别为38.9%、69.4%,2组治愈率和显效率比较差异有统计学意义(P<0.05)。治疗前2组IL-4水平均明显低于志愿组(P<0.01),IL-8明显高于志愿组(P<0.01)。治疗组治疗后IL-4水平明显上升,IL-8水平明显下降,与治疗前比较差异有统计学意义(P<0.01),2者水平与志愿组比较差异无统计学意义(P>0.05)。对照组治疗后IL-4水平有所上升,但与治疗前比较P>0.05,IL-8水平有明显下降,与治疗前比较P<0.01,IL-4上升幅度和IL-8下降幅度均不如治疗组显著(P<0.01)。[结论]健脾清肠法(中药健脾灵片加苦参槐花合剂)治疗UC的疗效明显优于西药艾迪莎,其机制可能与上调IL-4和下调IL-8水平,使机体免疫功能重新恢复平衡有关。

[Objective]To investigate clinical effects and influence of Jianpiqingchang recipe on interleukin-4 and interleukin-8 expression in serum of Ulcerative Colitis patients, [Methods]Seventy two cases with UC were divided randomly into two groups： treatment group and control group. The patients of treatment group accepted Jianpiling tablet and clyster of Kushenhuaihua treatment. The control group used Etiasa. All blood samples were taken before and after treatment to measure the level of interleukin-4（IL-4） and interleukin-8 （IL-8） by ELISA. [Results] The cure rate was 66. 7% and excellence effect rate was 91.7% in treatment group . The cure rate was 38. 9% and excellence effect rate was 69. 4% in control group. There were obvious differences between treatment group and control group in the rate of cure and excellence effect（P 〈0. 01） . Before treatment, the level of IL-4 was more lower than that in health persons（P〈 0. 01）, but the level of IL-8 was more higher in two groups（ P〈0. 01）. After treatment, the level of IL-4 clearly rose and the level of IL-8 clearly declined in treatment group（ P〈0. 01）. Therewere obvious differences compared with the level of IL-4 and IL-8 before treatment（P〈0. 01） , but no obvious difference with the level of health person. The level of IL-4 rose in control group, but there was no obvious difference compared with that before treatment. The level of IL8 clearly declined in control group after Etiasa treatment（P〈0. 01）. But the change of IL4 and IL8 in control group was lower than that in treatment group（P〈0. 01）. [Conclusion ]Therapeutic alliance with tablet of Jianpiling and clyster of Kushenhuaihua for UC was better than single treatment with Etiasa. The mechanism of therapeutic alliance may be through adjusting the level of IL-4 and IL8 and resuming the balance of immunologic function.