老年慢性阻塞性肺疾病患者TH_1与TH_2细胞亚群功能调控失衡的临床研究

Clinical research on the serum levels of interleukin-4,interferon-γ and interleukin-18 in elderly patiants with COPD

目的观察急性发作期以及缓解期的老年慢性阻塞性肺疾病(COPD)患者中IL-4、INF-γ与IL-18细胞因子水平表达变化。方法选择老年慢性支气管炎患者23例,采用竞争放射免疫法与双抗体夹心酶联免疫吸附法检测IL-4、INF-γ与IL-18水平变化,并进行急性发作期IL-4、INF-γ和IL-18水平与白细胞计数、分类的相关性分析。结果在COPD患者急性发作期的IL-4、INF-γ与IL-18水平均显著高于缓解期,而在缓解期仍然维持高水平状态,但三者与患者急性发作期白细胞中淋巴细胞、粒细胞计数、分类的无明显相关性。结论IL-4、IL-18与INF-γ参与COPD的气道炎症,而与白细胞数量变化无显著相关,提示COPD患者细胞因子网络调控有其特殊性。

Objective To observe the changs of the surem levels of IL-4, INF-γ and IL-18 in elderly patients with COPD. Methods Twenty three elderly patients with COPD and 15 healthy controls were enrolled in the clinical research. Levels of IL-4,INF-γ and IL-18 were detected by RIA and ELISA. Then the relationship between IL-4,INF-γ,IL-18 and differential white blood cell count was analyzed by statistic methods. Results The levels of IL-4.INF-γ and IL-18 were significantly higher at acute stage than in catabasis for elderly patiens with COPD;higer levels of them were still kept in catabosis. There were no significant correlations between the surem level of IL-18, INF-γ, IL-4 and differential white blood cell count at acute stage. Conclusion IL-4,INF-γ and IL-18 play important roles in the airway inflammation of elderly patients with COPD, and have no correlation with changes of differential white blood cell count, which suggests that there is a specific cytokines regurlation system in elderly patients with COPD.