白介素-18对人脐动脉平滑肌细胞组织因子表达的影响

Effects of Interleukin-18 on Expression of Tissue Factor in Cultured Human Umbilical Arterial Smooth Muscle Cells

目的:观察白介素-18(IL-18)对诱导体外培养的人脐动脉平滑肌细胞组织因子(TF)表达的作用。方法:在培养的人脐动脉平滑肌细胞中加入IL-18(浓度为100μg/L),用酶联免疫吸附分析法(ELISA)及细胞发色底物法分别测定培养基中TF抗原及细胞冻融液的TF活性。结果:培养基中TF抗原含量在4、6、8h组均高于24h组和对照组(P<0.05),且4h组最高(P<0.05),4h与6h2组间差别无统计学意义(P>0.05),8h组均低于4h组和6h组(P<0.05),24h组与对照组差别无统计学意义(P>0.05)。细胞冻融液中总TF活性在6、8、24h组均高于对照组(P<0.05),6h组和8h组均高于24h组(P<0.05),6h与8h组间差别无统计学意义(P>0.05)。结论:IL-18可以很快诱导体外培养的人脐动脉平滑肌细胞表达TF,使动脉血管平滑肌细胞呈现一过性促凝状态。

Objective： To investigate the effects of interleukin-18 （IL-18 ）on the expression of tissue factor （TF）in the cultured umbilical arterial smooth muscle cells. Methods： The cultured human umbilical arterial smooth muscle cells were incubated with IL-18 （100μg/L）. The tissue factor antigen in media was assayed by ELISA. The activity of rlT in cells lysates was estimated by chromogenic assay. Results： The antigen of TF in media was significantly higher in groups of 4 h, 6 h and 8 h than that in group of 24 h and controls （P〈 0.05）,the highest level in the group of 4 h （P〈 0.05）. There was no significant differences in antigen of TF between 4 h and 6 h groups （P 〉 0.05 ）and between groups of 24 h and control （P 〉 0.05）. The antigen of TF in media was significantly lower in group of 8 h than that in groups of 4 h and 6 h （P〈 0.05）. The activity of TF in cell lysates was significantly higher in groups of 6 h,8 h and 24 h than that in controls （P 〈 0.05）. The activity of TF was higher in groups of 6 h and 8 h compared with that in group of 24 h （P 〈 0.05）. There was no significant difference between groups 6 h and 8 h（P〉 0.05）. Conclusion＂ The tissue factor was rapidly induced in cultured umbilical arterial smooth muscle cells in vitro,which induce a transient procoagulant state on the surface of exposed smooth muscle cells.