醛固酮对人近端肾小管上皮细胞结缔组织生长因子mRNA表达的影响

Effect of Aldosterone on Expression of Connective Tissue Growth Factor in Human Proximal Tubular Epithelial Cells

目的:探讨醛固酮(ALD)在体外是否能够促进人近端肾小管上皮细胞株(HK2)结缔组织生长因子(CT-GF)的表达。方法:采用体外培养的HK2细胞,分别观察不同浓度(10-11、10-10、10-9、10-8、10-7mol/L)ALD处理48h,或用10-9mol/LALD处理不同时间(24h、48h、72h)对HK2细胞CTGF表达的影响,应用RT-PCR法检测CTGFmRNA水平的表达。结果:HK2细胞培养48h后,空白对照组即有CTGF的基础表达,ALD不同浓度组CTGFmRNA的表达均有增加,与空白对照组相比较差异有统计学意义(P<0.05或P<0.01);以10-9mol/L浓度的ALD作用于HK2细胞,随着作用时间的延长,CTGFmRNA的表达量明显增加,与对照组相比较差异有统计学意义(P<0.05或P<0.01)。结论:ALD能够促进HK2细胞CTGFmRNA水平的表达,并具有剂量依赖性和时间依赖性,ALD可能通过促进CTGF的分泌,发挥其促进肾间质纤维化的作用。

Objective： To investigate the effect of aldosterone （ALD）on expression of connective tissue growth factor （CTGF）in human proximal tubular epithelial ceils （HK2）. Methods： The human kidney ceil line was cultured for different times in presence or absence of different concentrations of ALD. The expression of CTGF mRNA responded to ALD was observed by RT-PCR. Results： There was a basal level expression of CTGF in normal condition. The expression of CTGF mRNA was significantly increased with ALD in a dose and time dependent manner. Conclusion： The expressin of CTGF mRNA was enhanced by ALD in a dose and time dependent manner. It is possible that ALD promote the progression of renal tubulointerstitial fibrosis by enhancing CTGF secretion.