三叉神经中脑核内假单极神经元的电生理和形态特性

ELECTROPHYSIOLOGICAL AND MORPHOLOGICAL PROPERTIES OF THE PSEUDOUNIPOLAR NEURONS IN MESENCEPHALIC TRIGEMINAL NUCLEUS

为了探讨三叉神经中脑核(Vme)神经元的电生理和形态学特征,本研究应用红外可视脑片膜片钳技术、biocytin细胞内标记技术以及免疫组织化学染色方法观察大鼠Vme内假单极神经元的电生理和形态学特征。结果显示:(1)长时程去极化方波刺激能够引起Vme神经元重复放电,且根据放电模式的不同,可将其分为快适应型神经元和慢适应型神经元两种类型,其中快适应型神经元占绝大多数;(2)经biocytin细胞内标记并染色后,光学显微镜下观察两种类型的Vme神经元在形态上均为假单极神经元,无显著差别。以上结果提示:形态学类型相同的Vme神经元的电生理学特点具有多样性,这可能与Vme神经元担负复杂的口面部本体觉信息的传递功能有关。

To explore the electrophysiological and morphological properties of the neurons in the mesencephalic trigeminal nucleus （Vme）, infrared visualized brain slice patch clamp combined with intracellular biocytin labeling and immunohistochemistry was employed to observe the electrophysiological properties and morphological characteristics of the pseudounipolar neurons of rat Vme. The present results showed that： （ 1 ） Repetitive neuronal discharges of Vme could be evoked by long term square wave depolarizing stimulus. The neurons could be divided into two types, rapid-adapting neurons and slow-adapting neurons, according to their different discharge patterns, and the majority of which was the rapid-adapting type; （2） There was no morphologically significant difference between such two types of Vme neurons which were labeled by the biocytin iontophoresed into Vme neuronal cell bodies and stained immunohistochamically. The present results suggest that morphologically identical Vme neurons may show diversified electrophysiological properties and may be involved into sophisticated functional transmission of oral-facial proprioceptive sensation.