大豆锈病与过氧化物酶同工酶关系的初步研究

PRELIMINARY STUDY ON THE CORRELATION BETWEEN SOYBEAN RUST INFECTION AND PEROXIDASE ISOENZYMES

采用聚丙烯酰胺凝胶电泳，研究了大豆锈病不同严重度、离病斑中心不同距离，以及抗感锈病不同品种接种后病程与过氧化物酶同工酶的关系，结果表明：发病不同严重度（Ⅰ～Ⅲ级）比对照（未接种０级）酶谱带多１～２条，活性由大到小依次为Ⅱ级＞Ⅰ级＞Ⅲ级＞０级；离病斑中心越近酶带越多，且酶活性越强；随着接种时间的推迟（０～３２小时）过氧化物酶带数增多，酶活性增强；抗病品种与感病品种接种后相同时间进行比较，抗病品种比感病品种多１～４条酶带，且其酶活性亦较高；Ｒｆ值０．７８～０．８５间的酶谱与大豆锈病的发生发展有着密切关系。

Using vertical gel electrophoresis, we studied the correlation between peroxidase isoenzyme and soybean rust of different severity and different distance apart from disease spots center and different time after inoculation in resistant and susceptible varieties. The results showed that there was one or two more isoenzyme pattern bands in different infection severity(from Ⅰ to Ⅲ grade) than CK treatment(Uninoculated, 0 grade). Peroxidase activity from high to low was Ⅱ grade>Ⅰ grade Ⅲ grade>0 grade. The closer distance apart from disease spots center and the period after inoculation in resistant and susceptible varieties the more stronger activity and the more enzyme pattern bands. Comparing resistant and susceptible varieties at the same time after inoculation, the resistant one had 1 to 4 more enzyme pattern bands and stronger enzyme activity than susceptible one. Besides there was close correlation between RF value (0.78 to 0.85) and development of soybean rust.